Journal article
Protein binding of iron in blood plasma of the ascidian Herdmania momus
Marine Biology, Vol.63(2), pp.107-112
1981
Abstract
Blood of the ascidian Herdmania momus var. grandii (Kott, 1952) has typically a low cell haematocrit volume, <1% with a cell density of 12 000 to 15 000 cells mm-3. Non-dialysable solids in the plasma are 105±10 mg%. Iron-binding in the plasma has been investigated by fractionation of 59Fe-labelled plasma, using gel chromatography and electrophoretic procedures. Three fractions, identified as I, II and III in the order of their elution from a G-150 column, bind 59Fe in the ratio 60:15:15. The dominant fraction, I, was shown by electrophoresis on polyacrylamide gels to consist of two components in the ratio of ca. 80:20 and with similar electrophoretic mobilities. Dissociation of Fraction I by mercaptoethanol and sodium dodecyl sulfate gave on electrophoresis three components of molecular weights 17 000; 32 000 and 66 000. Fraction 1 contained 10% carbohydrate by weight as determined by the ℓ-cysteine/sulphuric acid assay. These data are compared with other iron-binding systems such as transferrin and the siderophores. Phylogenetic aspects of the study are also discussed.
Details
- Title
- Protein binding of iron in blood plasma of the ascidian Herdmania momus
- Authors/Creators
- J. Webb (Author/Creator)P. Chrystal (Author/Creator)
- Publication Details
- Marine Biology, Vol.63(2), pp.107-112
- Publisher
- Springer Verlag
- Identifiers
- 991005543667407891
- Murdoch Affiliation
- School of Mathematical and Physical Sciences
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.22 Inorganic & Nuclear Chemistry
- 2.22.1857 Vanadium Biochemistry
- Web Of Science research areas
- Marine & Freshwater Biology
- ESI research areas
- Plant & Animal Science