Journal article
Proteome analysis of wheat leaf under salt stress by two-dimensional difference gel electrophoresis (2D-DIGE)
Phytochemistry, Vol.72(10), pp.1180-1191
2011
Abstract
Salt stress is a major abiotic stress that limits agricultural productivity in many regions of the world. To understand the molecular basis of the salt stress response in wheat (Triticum aestivum L.), a proteomic approach was used to identify the salt stress-responsive proteins in an elite Chinese wheat cultivar, Zhengmai 9023, which exhibits a high yield, superior gluten quality and better biotic resistance. Three-week-old seedlings were treated with NaCl of four different concentrations (1.0%, 1.5%, 2.0%, and 2.5%). The total proteins from the leaves of untreated and NaCl-treated plants were extracted and separated by two-dimensional difference gel electrophoresis (2D-DIGE). A total of 2358 protein spots were detected on the gels, among which 125 spots showed a significant change in protein abundance, and 83 differentially expressed spots were localised on preparative gels. Using Q-TOF mass spectrometry, 52 salt-responsive spots were identified, which were classified into six functional categories that included transport-associated proteins, detoxifying enzymes, ATP synthase, carbon metabolism, protein folding, and proteins with unknown biological functions. Of the 52 differentially expressed proteins, 26 were up-regulated, 21 were down-regulated, and five spots showed multi-expression patterns. In particular, some important proteins for salt tolerance were found to be up-regulated in Zhengmai 9023 under salt stress, such as H +-ATPases, glutathione S-transferase, ferritin and triosephosphate isomerase.
Details
- Title
- Proteome analysis of wheat leaf under salt stress by two-dimensional difference gel electrophoresis (2D-DIGE)
- Authors/Creators
- L. Gao (Author/Creator) - Capital Normal UniversityX. Yan (Author/Creator) - Capital Normal UniversityX. Li (Author/Creator) - Capital Normal UniversityG. Guo (Author/Creator) - Capital Normal UniversityY. Hu (Author/Creator) - Capital Normal UniversityW. Ma (Author/Creator) - Agriculture and FoodY. Yan (Author/Creator) - Capital Normal University
- Publication Details
- Phytochemistry, Vol.72(10), pp.1180-1191
- Publisher
- Elsevier BV
- Identifiers
- 991005540807607891
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.49 Plant Stress Responses
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Plant Sciences
- ESI research areas
- Plant & Animal Science