Proteome phenotypes discriminate the growing location and malting traits in Field-Grown Barley

M. Bahmani; A. Juhász; J. Broadbent; U. Bose; M.G. Nye-Wood; I.B. Edwards; M.L. Colgrave

doi:10.1021/acs.jafc.2c03816

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Proteome phenotypes discriminate the growing location and malting traits in Field-Grown Barley

Journal article

Open access

Peer reviewed

Proteome phenotypes discriminate the growing location and malting traits in Field-Grown Barley

M. Bahmani, A. Juhász, J. Broadbent, U. Bose, M.G. Nye-Wood, I.B. Edwards and M.L. Colgrave

Journal of Agricultural and Food Chemistry, Vol.70(34), pp.10680-10691

2022

DOI: https://doi.org/10.1021/acs.jafc.2c03816

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Abstract

Barley is one of the key cereal grains for malting and brewing industries. However, climate variability and unprecedented weather events can impact barley yield and end-product quality. The genetic background and environmental conditions are key factors in defining the barley proteome content and malting characteristics. Here, we measure the barley proteome and malting characteristics of three barley lines grown in Western Australia, differing in genetic background and growing location, by applying liquid chromatography–mass spectrometry (LC–MS). Using data-dependent acquisition LC–MS, 1571 proteins were detected with high confidence. Quantitative data acquired using sequential window acquisition of all theoretical (SWATH) MS on barley samples resulted in quantitation of 920 proteins. Multivariate analyses revealed that the barley lines’ genetics and their growing locations are strongly correlated between proteins and desired traits such as the malt yield. Linking meteorological data with proteomic measurements revealed how high-temperature stress in northern regions affects seed temperature tolerance during malting, resulting in a higher malt yield. Our results show the impact of environmental conditions on the barley proteome and malt characteristics; these findings have the potential to expedite breeding programs and malt quality prediction.

Details

Title: Proteome phenotypes discriminate the growing location and malting traits in Field-Grown Barley
Authors/Creators: M. Bahmani (Author/Creator) - Edith Cowan University
A. Juhász (Author/Creator) - Edith Cowan University
J. Broadbent (Author/Creator) - Commonwealth Scientific and Industrial Research Organisation
U. Bose (Author/Creator) - Edith Cowan University
M.G. Nye-Wood (Author/Creator) - Edith Cowan University
I.B. Edwards (Author/Creator) - Murdoch University
M.L. Colgrave (Author/Creator) - Edith Cowan University
Publication Details: Journal of Agricultural and Food Chemistry, Vol.70(34), pp.10680-10691
Publisher: American Chemical Society
Identifiers: 991005540060207891
Copyright: © 2022 The Authors.
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.249 Digestive System Disorders; 1.249.1112 Celiac Disease
Web Of Science research areas: Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology
ESI research areas: Agricultural Sciences