Proteomic analysis of albumins and globulins from wheat variety Chinese Spring and its fine deletion line 3BS-8

C-Y Ma; L-Y Gao; N. Li; X-H Li; W. Ma; R. Appels; Y-M Yan

doi:10.3390/ijms131013398

Back

Proteomic analysis of albumins and globulins from wheat variety Chinese Spring and its fine deletion line 3BS-8

Journal article

Open access

Peer reviewed

Proteomic analysis of albumins and globulins from wheat variety Chinese Spring and its fine deletion line 3BS-8

C-Y Ma, L-Y Gao, N. Li, X-H Li, W. Ma, R. Appels and Y-M Yan

International Journal of Molecular Sciences, Vol.13(10), pp.13398-13413

2012

DOI: https://doi.org/10.3390/ijms131013398

Files and links (2)

pdf

proteomic_analysis.pdfDownload View

Published (Version of Record) Open Access

url

Free to Read *No subscription requiredView

Abstract

The relationship between chromosome deletion in wheat and protein expression were investigated using Chinese Spring and fine deletion line 3BS-8. Through 2-DE (2-D electrophoresis) analysis, no differentially expressed proteins (DEPs) were found in leaf samples; however, 47 DEPs showed at least two-fold abundance variation (p < 0.05) in matured wheat grains and 21 spots were identified by tandem MALDI-TOF/TOF-MS. Among the identified spots, four were cultivar-specific, including three (spots B15, B16, and B21) in Chinese Spring and one in 3BS-8 (spot B10). Among variety-different DEPs between Chinese Spring and 3BS-8, most spots showed a higher express profile in CS; only four spots showed up-regulated expression tendency in 3BS-8. An interesting observation was that more than half of the identified protein spots were involved in storage proteins, of which 11 spots were identified as globulins. According to these results, we can presume that the encoded genes of protein spots B15, B16, and B21 were located on the chromosome segment deleted in 3BS-8.

Details

Title: Proteomic analysis of albumins and globulins from wheat variety Chinese Spring and its fine deletion line 3BS-8
Authors/Creators: C-Y Ma (Author/Creator) - Capital Normal University
L-Y Gao (Author/Creator)
N. Li (Author/Creator)
X-H Li (Author/Creator)
W. Ma (Author/Creator)
R. Appels (Author/Creator)
Y-M Yan (Author/Creator) - Capital Normal University
Publication Details: International Journal of Molecular Sciences, Vol.13(10), pp.13398-13413
Publisher: MDPI AG
Identifiers: 991005540695407891
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

226 File views/ downloads

70 Record Views

6 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
ESI research areas: Chemistry