Journal article
Proteomic evaluation of adults of Rhyzopertha dominica resistant to phosphine
Environmental Toxicology and Pharmacology, Vol.25(1), pp.121-126
2008
Abstract
A proteomic study using a PH3-susceptible (RD2) and -resistant strain (CRD343) of Rhyzopertha dominica was undertaken to validate the relation between change of proteins and PH3 resistance. Protein expression levels were compared using PD-Quest program after two-dimensional polyacrylamide gel-electrophoresis. Comparing the intensity of proteins, 15 proteins decreased and 6 proteins were newly expressed in CRD343. After MALDI-TOF and LC-MS/MS analyses, the decreased proteins were identified as arginine kinases, dihydrolipoamide dehydrogenase, Hsp60, reverse transcriptase, glyceraldehydes-3-phosphate dehydrogenase, triosephosphate isomerase and hypothetical proteins. Dihydrolipoamide dehydrogenase is involved in the Krebs cycle and glyceraldehydes-3-phosphate dehydrogenase and triosephosphate isomerase are involved in the glycolysis pathway. Among up-regulated proteins, sodium channel, glutamate racemase, enolase and vitellogenin were identified. Taken together, PH3 affected glycolysis as well as Krebs cycle and the induction of enolase might recover this dysfunction.
Details
- Title
- Proteomic evaluation of adults of Rhyzopertha dominica resistant to phosphine
- Authors/Creators
- B-S Park (Author/Creator)B-H Lee (Author/Creator)T-W Kim (Author/Creator)Y. Ren (Author/Creator)S-E Lee (Author/Creator)
- Publication Details
- Environmental Toxicology and Pharmacology, Vol.25(1), pp.121-126
- Publisher
- Elsevier B.V.
- Identifiers
- 991005544488107891
- Copyright
- © 2007 Elsevier B.V.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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