Proteomic profiling of developing wheat heads under water-stress

S. Islam; P. Wang; D. Vincent; J.M. Khan; A. Juhász; D. Diepeveen; R.J. Lipscombe; W. Ma; R. Appels

doi:10.1007/s10142-020-00746-9

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Proteomic profiling of developing wheat heads under water-stress

Journal article

Peer reviewed

Proteomic profiling of developing wheat heads under water-stress

S. Islam, P. Wang, D. Vincent, J.M. Khan, A. Juhász, D. Diepeveen, R.J. Lipscombe, W. Ma and R. Appels

Functional & Integrative Genomics, Vol.20, pp.695-710

2020

DOI: https://doi.org/10.1007/s10142-020-00746-9

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Abstract

A replicated iTRAQ (isobaric tags for relative and absolute quantification) study on developing wheat heads from two doubled haploid (DH) lines identified from a cross between cv Westonia x cv Kauz characterized the proteome changes influenced by reproductive stage water-stress. All lines were exposed to 10 days of water-stress from early booting (Zadok 40), with sample sets taken from five head developmental stages. Two sample groups (water-stressed and control) account for 120 samples that required 18 eight-plex iTRAQ runs. Based on the IWGSC RefSeq v1 wheat assembly, among the 4592 identified proteins, a total of 132 proteins showed a significant response to water-stress, including the down-regulation of a mitochondrial Rho GTPase, a regulator of intercellular fundamental biological processes (7.5 fold) and cell division protein FtsZ at anthesis (6.0 fold). Up-regulated proteins included inosine-5′-monophosphate dehydrogenase (3.83 fold) and glycerophosphodiester phosphodiesterase (4.05 fold). The Pre-FHE and FHE stages (full head emerged) of head development were differentiated by 391 proteins and 270 proteins differentiated the FHE and Post-FHE stages. Water-stress during meiosis affected seed setting with 27% and 6% reduction in the progeny DH105 and DH299 respectively. Among the 77 proteins that differentiated between the two DH lines, 7 proteins were significantly influenced by water-stress and correlated with the seed set phenotype response of the DH lines to water-stress (e.g. the up-regulation of a subtilisin-like protease in DH 299 relative to DH 105). This study provided unique insights into the biological changes in developing wheat head that occur during water-stress.

Details

Title: Proteomic profiling of developing wheat heads under water-stress
Authors/Creators: S. Islam (Author/Creator) - Murdoch University
P. Wang (Author/Creator) - Murdoch University
D. Vincent (Author/Creator) - Agriculture Victoria
J.M. Khan (Author/Creator) - HCI International Medical Centre
A. Juhász (Author/Creator) - Edith Cowan University
D. Diepeveen (Author/Creator) - Government of Western Australia
R.J. Lipscombe (Author/Creator) - HCI International Medical Centre
W. Ma (Author/Creator) - Murdoch University
R. Appels (Author/Creator) - The University of Melbourne
Publication Details: Functional & Integrative Genomics, Vol.20, pp.695-710
Publisher: Springer
Identifiers: 991005540878207891
Murdoch Affiliation: College of Science, Health, Engineering and Education
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.49 Plant Stress Responses
Web Of Science research areas: Genetics & Heredity
ESI research areas: Molecular Biology & Genetics