Journal article
Proteomic profiling of developing wheat heads under water-stress
Functional & Integrative Genomics, Vol.20, pp.695-710
2020
Abstract
A replicated iTRAQ (isobaric tags for relative and absolute quantification) study on developing wheat heads from two doubled haploid (DH) lines identified from a cross between cv Westonia x cv Kauz characterized the proteome changes influenced by reproductive stage water-stress. All lines were exposed to 10 days of water-stress from early booting (Zadok 40), with sample sets taken from five head developmental stages. Two sample groups (water-stressed and control) account for 120 samples that required 18 eight-plex iTRAQ runs. Based on the IWGSC RefSeq v1 wheat assembly, among the 4592 identified proteins, a total of 132 proteins showed a significant response to water-stress, including the down-regulation of a mitochondrial Rho GTPase, a regulator of intercellular fundamental biological processes (7.5 fold) and cell division protein FtsZ at anthesis (6.0 fold). Up-regulated proteins included inosine-5′-monophosphate dehydrogenase (3.83 fold) and glycerophosphodiester phosphodiesterase (4.05 fold). The Pre-FHE and FHE stages (full head emerged) of head development were differentiated by 391 proteins and 270 proteins differentiated the FHE and Post-FHE stages. Water-stress during meiosis affected seed setting with 27% and 6% reduction in the progeny DH105 and DH299 respectively. Among the 77 proteins that differentiated between the two DH lines, 7 proteins were significantly influenced by water-stress and correlated with the seed set phenotype response of the DH lines to water-stress (e.g. the up-regulation of a subtilisin-like protease in DH 299 relative to DH 105). This study provided unique insights into the biological changes in developing wheat head that occur during water-stress.
Details
- Title
- Proteomic profiling of developing wheat heads under water-stress
- Authors/Creators
- S. Islam (Author/Creator) - Murdoch UniversityP. Wang (Author/Creator) - Murdoch UniversityD. Vincent (Author/Creator) - Agriculture VictoriaJ.M. Khan (Author/Creator) - HCI International Medical CentreA. Juhász (Author/Creator) - Edith Cowan UniversityD. Diepeveen (Author/Creator) - Government of Western AustraliaR.J. Lipscombe (Author/Creator) - HCI International Medical CentreW. Ma (Author/Creator) - Murdoch UniversityR. Appels (Author/Creator) - The University of Melbourne
- Publication Details
- Functional & Integrative Genomics, Vol.20, pp.695-710
- Publisher
- Springer
- Identifiers
- 991005540878207891
- Murdoch Affiliation
- College of Science, Health, Engineering and Education
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.49 Plant Stress Responses
- Web Of Science research areas
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics