Journal article
Psoriasis-Specific RNA Isoforms Identified by RNA-Seq Analysis of 173,446 Transcripts
Frontiers in Medicine, Vol.3
2016
Abstract
Background: Several studies have been published that investigated potential links between transcriptome changes and psoriasis using microarrays and RNA-seq technologies, but no previous study has analyzed expression profile of alternatively spliced transcripts in psoriasis.
Objectives: Identification of potential alternatively spliced RNA isoforms with disease-specific expression profile.
Methods: Using our published RNA sequencing data from lesional psoriatic (LP), non-lesional psoriatic (NLP), and normal control skin (C), we analyzed the differential expression of RNA splicing variants. LP sample was compared with NLP, as was LP with C and NLP with C.
Results: Transcript-based annotation analyzed 173,446 transcripts (RNA isoforms), and around 9,000 transcripts were identified as differentially expressed between study groups. Several previously undescribed RNA variants were found. For instance, transcript ETV3_3 (ENST00000326786) was significantly downregulated in LP and NLP skin. ETV3 is a transcriptional repressor that contributes to the downstream anti-inflammatory effects of IL-10. We also identified diseases-specific transcripts (S100A7A, IL36RN_4, and IL36G_3) of genes already recognized to be involved in inflammation and immune response.
Conclusion: Psoriasis is characterized by significant differences in the expression of RNA alternative isoforms. Description of these new isoforms improves our knowledge about this complex disease.
Details
- Title
- Psoriasis-Specific RNA Isoforms Identified by RNA-Seq Analysis of 173,446 Transcripts
- Authors/Creators
- S. Kõks (Author/Creator) - University of TartuM. Keermann (Author/Creator) - Tartu University HospitalE. Reimann (Author/Creator) - University of TartuE. Prans (Author/Creator) - University of TartuK. Abram (Author/Creator) - Tartu University HospitalH. Silm (Author/Creator) - Tartu University HospitalG. Kõks (Author/Creator) - University of TartuK. Kingo (Author/Creator) - Tartu University Hospital
- Publication Details
- Frontiers in Medicine, Vol.3
- Publisher
- Frontiers Media
- Identifiers
- 991005540182607891
- Copyright
- © 2016 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.106 Rheumatology
- 1.106.737 Psoriasis
- Web Of Science research areas
- Dermatology
- ESI research areas
- Clinical Medicine