Pulmonary delivery of cationic gold nanoparticles boost antigen-specific CD4+ T Cell Proliferation

E. Seydoux; L. Rodriguez-Lorenzo; R.A.M. Blom; P.A. Stumbles; A. Petri-Fink; B.M. Rothen-Rutishauser; F. Blank; C. von Garnier

doi:10.1016/j.nano.2016.02.020

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Pulmonary delivery of cationic gold nanoparticles boost antigen-specific CD4+ T Cell Proliferation

Journal article

Open access

Peer reviewed

Pulmonary delivery of cationic gold nanoparticles boost antigen-specific CD4+ T Cell Proliferation

E. Seydoux, L. Rodriguez-Lorenzo, R.A.M. Blom, P.A. Stumbles, A. Petri-Fink, B.M. Rothen-Rutishauser, F. Blank and C. von Garnier

Nanomedicine: Nanotechnology, Biology and Medicine, Vol.12(7), pp.1815-1826

2016

DOI: https://doi.org/10.1016/j.nano.2016.02.020

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Abstract

To address how surface charge affects the fate of potential nanocarriers in the lung, gold nanoparticles (AuNPs) coated with polyvinyl alcohol containing either positively (NH2) or negatively (COOH) charged functional groups were intra-nasally instilled in mice, and their uptake by antigen presenting cell populations (APC) in broncho-alveolar lavage (BAL) fluid, trachea, and lung parenchyma, as well as trafficking to the lung draining lymph nodes (LDLNs) was assessed by flow cytometry. Furthermore, CD4+ T cell proliferation in LDLNs was investigated following instillation. All APC subpopulations preferentially captured positively-charged AuNPs compared to their negatively-charged counterparts. Uptake of AuNPs up-regulated expression of co-stimulatory molecules on all APC populations. Furthermore, positively-charged AuNPs induced enhanced OVA-specific CD4+ T cell stimulation in LDLNs compared to negatively-charged AuNPs, or polymer alone. Our findings demonstrate surface charge as a key parameter determining particle uptake by APC, and down-stream immune responses depend on the presence of particle core-bound polymer.

Details

Title: Pulmonary delivery of cationic gold nanoparticles boost antigen-specific CD4+ T Cell Proliferation
Authors/Creators: E. Seydoux (Author/Creator) - University Hospital of Bern
L. Rodriguez-Lorenzo (Author/Creator) - Adolphe Merkle Institute
R.A.M. Blom (Author/Creator) - University Hospital of Bern
P.A. Stumbles (Author/Creator) - Murdoch University
A. Petri-Fink (Author/Creator) - Adolphe Merkle Institute
B.M. Rothen-Rutishauser (Author/Creator) - Adolphe Merkle Institute
F. Blank (Author/Creator) - University Hospital of Bern
C. von Garnier (Author/Creator) - University Hospital of Bern
Publication Details: Nanomedicine: Nanotechnology, Biology and Medicine, Vol.12(7), pp.1815-1826
Publisher: Elsevier
Identifiers: 991005544939907891
Copyright: © 2016 Elsevier Inc.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.6 Immunology; 1.6.358 Dendritic Cell Therapy
Web Of Science research areas: Medicine, Research & Experimental; Nanoscience & Nanotechnology
ESI research areas: Clinical Medicine