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CC BY V4.0, Open Access
Journal article
Pyruvate Regulates the Expression of DLAT to Promote Follicular Growth
Cells (Basel, Switzerland), Vol.14(6), 444
2025
PMID: 40136693
Abstract
Increasing evidence has suggested that dihydrolipoamide S-acetyltransferase (DLAT), a subunit of the pyruvate dehydrogenase complex, is crucial for pyruvate metabolism and the regulation of cell death. The excessive death of granulosa cells (GCs) hinders the progression of follicular growth. However, the relationship between DLAT and follicular growth is poorly understood. Here, we found that pyruvate significantly shortened the age of pubertal initiation in mice and promoted follicular growth by promoting the proliferation of GCs. In addition, pyruvate up-regulated the expression of DLAT and the high level of DLAT was observed in large follicles, which were associated with follicular growth. Mechanistically, DLAT increased the mRNA and protein levels of proliferation pathways such as PCNA and MCL1 to promote GC proliferation. Additionally, DLAT bound to CASP3 and CASP9 proteins to inhibit the apoptosis of GCs. Taken together, these results reveal a mechanism that pyruvate regulated DLAT to promote follicular growth, and DLAT represents a promising target that supports new strategies for improving the growth of follicles.
Details
- Title
- Pyruvate Regulates the Expression of DLAT to Promote Follicular Growth
- Authors/Creators
- Liuhong Zhang - State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaYixuan Guo - South China Agricultural UniversityEnyuan Huang - State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaJianing Lu - State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaTiantian Wang - South China Agricultural UniversityYonghua Shi - Agricultural Genomics Institute at ShenzhenMeng Lv - South China Agricultural UniversityYongcai Chen - South China Agricultural UniversityShuo Li - South China Agricultural UniversityXiaolong Yuan - South China Agricultural UniversityJiaqi Li - South China Agricultural University
- Publication Details
- Cells (Basel, Switzerland), Vol.14(6), 444
- Publisher
- MDPI
- Number of pages
- 17
- Grant note
- 2024B1515020112, 2024A1515012999, 2023A1515030054 / Guangdong Basic and Applied Basic Research Foundation 31902131 and 32072694 / the National Natural Science Foundation of China CARS-35 / Earmarked fund for China Agriculture Research System
- Identifiers
- 991005753924407891
- Copyright
- © 2025 by the authors.
- Murdoch Affiliation
- Centre for Healthy Ageing
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.197 Molecular & Cell Biology - Mitochondria
- 1.197.2017 Lipoic Acid Metabolism
- Web Of Science research areas
- Cell Biology
- ESI research areas
- Biology & Biochemistry