Journal article
Quantification and localization of formylated phloroglucinol compounds (FPCs) in eucalyptus species
Frontiers in Plant Science, Vol.10, Article 186
2019
Abstract
The Eucalyptus genus is a hyper-diverse group of long-lived trees from the Myrtaceae family, consisting of more than 700 species. Eucalyptus are widely distributed across their native Australian landscape and are the most widely planted hardwood forest trees in the world. The ecological and economic success of Eucalyptus trees is due, in part, to their ability to produce a plethora of specialized metabolites, which moderate abiotic and biotic interactions. Formylated phloroglucinol compounds (FPCs) are an important class of specialized metabolites in the Myrtaceae family, particularly abundant in Eucalyptus. FPCs are mono- to tetra-formylated phloroglucinol based derivatives, often with an attached terpene moiety. These compounds provide chemical defense against herbivory and display various bioactivities of pharmaceutical relevance. Despite their ecological and economic importance, and continued improvements into analytical techniques, FPCs have proved challenging to study. Here we present a simple and reliable method for FPCs extraction, identification and quantification by UHPLC-DAD-ESI-Q-TOF-MS/MS. The method was applied to leaf, flower bud, and flower samples of nine different eucalypt species, using a small amount of plant material. Authentic analytical standards were used to provide high resolution mass spectra and fragmentation patterns. A robust method provides opportunities for future investigations into the identification and quantification of FPCs in complex biological samples with high confidence. Furthermore, we present for the first time the tissue-based localization of FPCs in stem, leaf, and flower bud of Eucalyptus species measured by mass spectrometry imaging, providing important information for biosynthetic pathway discovery studies and for understanding the role of those compounds in planta.
Details
- Title
- Quantification and localization of formylated phloroglucinol compounds (FPCs) in eucalyptus species
- Authors/Creators
- B.M. Santos (Author/Creator)J.F.S. Zibrandtsen (Author/Creator) - University of CopenhagenD. Gunbilig (Author/Creator) - University of CopenhagenM. Sørensen (Author/Creator) - University of CopenhagenF. Cozzi (Author/Creator) - University of CopenhagenB.A. Boughton (Author/Creator) - The University of MelbourneA.M. Heskes (Author/Creator) - University of CopenhagenE.H.J. Neilson (Author/Creator) - University of Copenhagen
- Publication Details
- Frontiers in Plant Science, Vol.10, Article 186
- Publisher
- Frontiers Media
- Identifiers
- 991005545516307891
- Copyright
- © 2019 Santos, Zibrandtsen, Gunbilig, Sørensen, Cozzi, Boughton, Heskes and Neilson
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.16 Phytochemicals
- 3.16.1867 Plant Xanthones
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science