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Journal article
Quantifying citrate-enhanced phosphate root uptake using microdialysis
Plant and soil, Vol.461(1-2), pp.69-89
2021
PMID: 34720207
Abstract
Aims
Organic acid exudation by plant roots is thought to promote phosphate (P) solubilisation and bioavailability in soils with poorly available nutrients. Here we describe a new combined experimental (microdialysis) and modelling approach to quantify citrate-enhanced P desorption and its importance for root P uptake.
Methods
To mimic the rhizosphere, microdialysis probes were placed in soil and perfused with citrate solutions (0.1, 1.0 and 10 mM) and the amount of P recovered from soil used to quantify rhizosphere P availability. Parameters in a mathematical model describing probe P uptake, citrate exudation, P movement and citrate-enhanced desorption were fit to the experimental data. These parameters were used in a model of a root which exuded citrate and absorbed P. The importance of soil citrate-P mobilisation for root P uptake was then quantified using this model.
Results
A plant needs to exude citrate at a rate of 0.73 μmol cm−1 of root h−1 to see a significant increase in P absorption. Microdialysis probes with citrate in the perfusate were shown to absorb similar quantities of P to an exuding root.
Conclusion
A single root exuding citrate at a typical rate (4.3 × 10−5 μmol m−1 of root h−1) did not contribute significantly to P uptake. Microdialysis probes show promise for measuring rhizosphere processes when calibration experiments and mathematical modelling are used to decouple microdialysis and rhizosphere mechanisms.
Details
- Title
- Quantifying citrate-enhanced phosphate root uptake using microdialysis
- Authors/Creators
- D. M. McKay Fletcher - University of SouthamptonR. Shaw - Bangor UniversityA. R. Sanchez-Rodriguez - Bangor UniversityK. R. Daly - University of SouthamptonA. van Veelen - University of SouthamptonD. L. Jones - Bangor UniversityT. Roose - University of Southampton
- Publication Details
- Plant and soil, Vol.461(1-2), pp.69-89
- Publisher
- Springer Nature
- Number of pages
- 21
- Grant note
- EP/M020355/1 / EPSRC; UK Research & Innovation (UKRI); Engineering & Physical Sciences Research Council (EPSRC) BB/L025620/1; BB/I024283/1 / BBSRC; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC) BB/L025620/1 / BBSRC SARISA; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC) NE/L00237/1 / NERC; UK Research & Innovation (UKRI); Natural Environment Research Council (NERC) 646,809 / ERC Consolidator grant; European Research Council (ERC) BB/P004180/1 / BBSRC SARIC; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC)
- Identifiers
- 991005560338507891
- Copyright
- © 2019 The Author(s)
- Murdoch Affiliation
- Centre for Sustainable Farming Systems
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.45 Soil Science
- 3.45.473 Soil Phosphorus Dynamics
- Web Of Science research areas
- Agronomy
- Plant Sciences
- Soil Science
- ESI research areas
- Agricultural Sciences