Quantitation of muscle precursor cell activity in skeletal muscle by Northern analysis of MyoD and myogenin expression: Application to dystrophic (mdx) mouse muscle

M.W. Beilharz; R.R. Lareu; K.L. Garrett; M.D. Grounds; S. Fletcher

doi:10.1016/1044-7431(92)90029-2

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Quantitation of muscle precursor cell activity in skeletal muscle by Northern analysis of MyoD and myogenin expression: Application to dystrophic (mdx) mouse muscle

Journal article

Peer reviewed

Quantitation of muscle precursor cell activity in skeletal muscle by Northern analysis of MyoD and myogenin expression: Application to dystrophic (mdx) mouse muscle

M.W. Beilharz, R.R. Lareu, K.L. Garrett, M.D. Grounds and S. Fletcher

Molecular and Cellular Neuroscience, Vol.3(4), pp.326-331

1992

DOI: https://doi.org/10.1016/1044-7431(92)90029-2

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Abstract

The regeneration of skeletal muscle is dependent upon proliferation and fusion of activated mononuclear muscle precursor cells. Early and specific markers of this population of activated cells are the transcription factors MyoD and myogenin. Northern analysis was used to determine levels of MyoD and myogenin mRNA in (i) muscles regenerating after experimental crush injury and (ii) in limb muscles of dystrophic mdx mice at various ages in comparison to controls. In crush-injured muscle, MyoD and myogenin mRNA increased at 24 h, peaked between 2 to 6 days, and returned to uninjured control levels by 15 days after injury. In both mdx and control mice, MyoD and myogenin mRNA levels were high in fetal muscles and decreased rapidly during the 2 weeks after birth. In mdx muscles, the mRNA levels increased significantly from about 21 days, remained high until around 40 days, and then decreased to a relatively constant yet elevated level when compared to control muscles. The elevated levels persisted to 420 days of age. The results show that this technique can be used to provide sensitive quantitative information on the size of the population of activated precursor cells in skeletal muscle. As such, it represents a novel and convenient means of measuring regenerative activity in vivo in whole muscles.

Details

Title: Quantitation of muscle precursor cell activity in skeletal muscle by Northern analysis of MyoD and myogenin expression: Application to dystrophic (mdx) mouse muscle
Authors/Creators: M.W. Beilharz (Author/Creator) - The University of Western Australia
R.R. Lareu (Author/Creator) - The University of Western Australia
K.L. Garrett (Author/Creator) - The University of Western Australia
M.D. Grounds (Author/Creator) - School of Anatomy, Physiology and Human Biology
S. Fletcher (Author/Creator) - Centre for Neuromuscular and Neurological Disorders
Publication Details: Molecular and Cellular Neuroscience, Vol.3(4), pp.326-331
Publisher: Elsevier
Identifiers: 991005541121707891
Copyright: © 1992 Published by Academic Press Inc.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.255 Musculoskeletal Disorders; 1.255.628 Duchenne Muscular Dystrophy
Web Of Science research areas: Neurosciences
ESI research areas: Neuroscience & Behavior