Quantitative Variation in Effector Activity of ToxA Isoforms fromStagonospora nodorumandPyrenophora tritici-repentis

K.C. Tan; M. Ferguson-Hunt; K. Rybak; O.D.C. Waters; W.A. Stanley; C.S. Bond; E.H. Stukenbrock; T.L. Friesen; J.D. Faris; B.A. McDonald; R.P. Oliver

doi:10.1094/MPMI-10-11-0273

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Quantitative Variation in Effector Activity of ToxA Isoforms fromStagonospora nodorumandPyrenophora tritici-repentis

Journal article

Peer reviewed

Quantitative Variation in Effector Activity of ToxA Isoforms fromStagonospora nodorumandPyrenophora tritici-repentis

K.C. Tan, M. Ferguson-Hunt, K. Rybak, O.D.C. Waters, W.A. Stanley, C.S. Bond, E.H. Stukenbrock, T.L. Friesen, J.D. Faris, B.A. McDonald, …

Molecular Plant-Microbe Interactions, Vol.25(4), pp.515-522

2012

DOI: https://doi.org/10.1094/MPMI-10-11-0273

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Abstract

ToxA is a proteinaceous necrotrophic effector produced by Stagonospora nodorum and Pyrenophora tritici-repentis. In this study, all eight mature isoforms of the ToxA protein were purified and compared. Circular dichroism spectra indicated that all isoforms were structurally intact and had indistinguishable secondary structural features. ToxA isoforms were infiltrated into wheat lines that carry the sensitivity gene Tsn1. It was observed that different wheat lines carrying identical Tsn1 alleles varied in sensitivity to ToxA. All ToxA isoforms induced necrosis when introduced into any Tsn1 wheat line but we observed quantitative variation in effector activity, with the least-active version found in isolates of P. tritici-repentis. Pathogen sporulation increased with higher doses of ToxA. The isoforms that induced the most rapid necrosis also induced the most sporulation, indicating that pathogen fitness is affected by differences in ToxA activity. We show that differences in toxin activity encoded by a single gene can contribute to the quantitative inheritance of necrotrophic virulence. Our findings support the hypothesis that the variation at ToxA results from selection that favors increased toxin activity.

Details

Title: Quantitative Variation in Effector Activity of ToxA Isoforms fromStagonospora nodorumandPyrenophora tritici-repentis
Authors/Creators: K.C. Tan (Author/Creator) - Curtin University
M. Ferguson-Hunt (Author/Creator) - Murdoch University
K. Rybak (Author/Creator) - Murdoch University
O.D.C. Waters (Author/Creator) - Curtin University
W.A. Stanley (Author/Creator) - ARC Centre of Excellence in Plant Energy Biology
C.S. Bond (Author/Creator) - School of Molecular Sciences
E.H. Stukenbrock (Author/Creator) - Max Planck Society
T.L. Friesen (Author/Creator) - United States Dept. of Agriculture
J.D. Faris (Author/Creator) - United States Dept. of Agriculture
B.A. McDonald (Author/Creator) - École Polytechnique Fédérale de Lausanne
R.P. Oliver (Author/Creator) - Curtin University
Publication Details: Molecular Plant-Microbe Interactions, Vol.25(4), pp.515-522
Publisher: American Phytopathological Society
Identifiers: 991005541450507891
Copyright: © 2012 The American Phytopathological Society
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.97 Plant Pathology; 3.97.636 Fungal Plant Pathogens
Web Of Science research areas: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences
ESI research areas: Plant & Animal Science