Journal article
Quantitative real-time PCR in aDNA research
Ancient DNA, Vol.840, pp.121-132
2012
Abstract
Quantitative real-time PCR (qPCR) is a technique that is widely used in the field of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing. In this chapter, we outline factors that need to be considered when developing efficient SYBR Green qPCR assays. We describe how to setup qPCR standards of known copy number and provide some useful tips regarding interpretation of qPCR data generated from aDNA templates.
Details
- Title
- Quantitative real-time PCR in aDNA research
- Authors/Creators
- M. Bunce (Author/Creator) - Murdoch UniversityC.L. Oskam (Author/Creator) - Murdoch UniversityM.E. Allentoft (Author/Creator) - Murdoch University
- Publication Details
- Ancient DNA, Vol.840, pp.121-132
- Publisher
- Humana Press, Inc.
- Identifiers
- 991005541649807891
- Copyright
- © 2012 Springer Science+Business Media, LLC.
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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