RAPD (random amplified polymorphic DNA) analysis of Giardia DNA and correlation with isoenzyme data

U.M. Morgan; C.C. Constantine; W.K. Greene; R.C.A. Thompson

doi:10.1016/0035-9203(93)90303-8

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RAPD (random amplified polymorphic DNA) analysis of Giardia DNA and correlation with isoenzyme data

Journal article

Peer reviewed

RAPD (random amplified polymorphic DNA) analysis of Giardia DNA and correlation with isoenzyme data

U.M. Morgan, C.C. Constantine, W.K. Greene and R.C.A. Thompson

Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol.87(6), pp.702-705

1993

DOI: https://doi.org/10.1016/0035-9203(93)90303-8

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Abstract

Fourteen Giardia duodenalis isolates were examined using the RAPD (random amplified polymorphic deoxyribonucleic acid) technique. Simple reproducible polymorphisms were generated using 3 different RAPD primers. The results generated by each primer were very similar and were significantly correlated with each other. These data were then compared to existing isoenzyme electrophoresis data on the same isolates. The RAPD data divided the isolates into 10 groupings or rapdemes while the isoenzyme data divided them into 10 similar zymodemes. Both methods grouped 4 isolates (BAH42, BAH44c9, BAH12c9 and BAH39c7), which comprised a phenotypically heterogeneous assemblage with respect to growth rate and metabolism, into similar groupings. The 2 methods were significantly correlated (P<0.001). It will therefore be possible to use RAPD for the characterization of isolates of Giardia, and other parasites such as Cryprosporidium, which are refractory to cultivation in vitro.

Details

Title: RAPD (random amplified polymorphic DNA) analysis of Giardia DNA and correlation with isoenzyme data
Authors/Creators: U.M. Morgan (Author/Creator) - Murdoch University
C.C. Constantine (Author/Creator) - Murdoch University
W.K. Greene (Author/Creator) - Murdoch University
R.C.A. Thompson (Author/Creator)
Publication Details: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol.87(6), pp.702-705
Publisher: Royal Society of Tropical Medicine and Hygiene
Identifiers: 991005545999107891
Copyright: © 1993 Published by Elsevier Ltd
Murdoch Affiliation: School of Veterinary Studies
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Public, Environmental & Occupational Health; Tropical Medicine
ESI research areas: Social Sciences, general