RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II

M. Zaratiegui; S.E. Castel; D.V. Irvine; A. Kloc; J. Ren; F. Li; E. de Castro; L. Marín; A-Y Chang; D.B. Goto; W.Z. Cande; F. Antequera; B. Arcangioli; R. A. Martienssen

doi:10.1038/nature10501

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RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II

Journal article

Peer reviewed

RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II

M. Zaratiegui, S.E. Castel, D.V. Irvine, A. Kloc, J. Ren, F. Li, E. de Castro, L. Marín, A-Y Chang, D.B. Goto, …

Nature, Vol.479(7371), pp.135-138

2011

DOI: https://doi.org/10.1038/nature10501

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Abstract

Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification during the DNA replication phase of the cell cycle. Here we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication in Schizosaccharomyces pombe. Co-transcriptional RNAi releases RNA polymerase II (Pol II), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes that spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification.

Details

Title: RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II
Authors/Creators: M. Zaratiegui (Author/Creator) - Cold Spring Harbor Laboratory
S.E. Castel (Author/Creator) - Cold Spring Harbor Laboratory
D.V. Irvine (Author/Creator) - Cold Spring Harbor Laboratory
A. Kloc (Author/Creator) - Cold Spring Harbor Laboratory
J. Ren (Author/Creator) - Cold Spring Harbor Laboratory
F. Li (Author/Creator) - University of California, Berkeley
E. de Castro (Author/Creator) - Instituto de Biología Funcional y Genómica
L. Marín (Author/Creator) - Instituto de Biología Funcional y Genómica
A-Y Chang (Author/Creator) - Cold Spring Harbor Laboratory
D.B. Goto (Author/Creator) - Cold Spring Harbor Laboratory
W.Z. Cande (Author/Creator) - University of California, Berkeley
F. Antequera (Author/Creator) - Instituto de Biología Funcional y Genómica
B. Arcangioli (Author/Creator) - Cold Spring Harbor Laboratory
R. A. Martienssen (Author/Creator) - Cold Spring Harbor Laboratory
Publication Details: Nature, Vol.479(7371), pp.135-138
Publisher: Nature Publishing Group
Identifiers: 991005540964707891
Copyright: © 2011 Macmillan Publishers Limited.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.54 Molecular & Cell Biology - Genetics; 1.54.100 Epigenetic Regulation
Web Of Science research areas: Multidisciplinary Sciences
ESI research areas: Molecular Biology & Genetics