RNase E levels in Escherichia coli are controlled by a complex regulatory system that involves transcription of the rne gene from three promoters

M.C. Ow; Q. Liu; B.K. Mohanty; M.E. Andrew; V.F. Maples; S.R. Kushner

doi:10.1046/j.1365-2958.2002.02726.x

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RNase E levels in Escherichia coli are controlled by a complex regulatory system that involves transcription of the rne gene from three promoters

Journal article

Peer reviewed

RNase E levels in Escherichia coli are controlled by a complex regulatory system that involves transcription of the rne gene from three promoters

M.C. Ow, Q. Liu, B.K. Mohanty, M.E. Andrew, V.F. Maples and S.R. Kushner

Molecular Microbiology, Vol.43(1), pp.159-171

2002

DOI: https://doi.org/10.1046/j.1365-2958.2002.02726.x

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Abstract

The rne gene of Escherichia coli encodes RNase E, an essential endoribonuclease that is involved in both mRNA decay and rRNA processing. Here we present evidence that the gene is transcribed from three promoters: p1, p2 and p3. The p2 and p3 promoters map 34 and 145 nt upstream from the previously characterized rne promoter, p1, generating unusually long 5′ UTRs of 395 and 506 nt respectively. Based on promoter-lacZ transcriptional fusions, p1 is a more efficient promoter than either p2 or p3. Low copy number or single copy number vectors carrying rne transcribed from either p1, p2 or p3 alone complement the rneΔ1018::bla deletion mutation at 30°C, 37°C and 44°C. However, normal autoregulation requires the presence of all three promoters. A comparison among intracellular levels of RNase E, the half-lives of the rpsO, rpsT and rne mRNAs, and growth rates, indicates that the cell contains a considerable excess of RNase E protein. In addition, when the rne transcript is stabilized at low RNase E levels, it is not efficiently translated.

Details

Title: RNase E levels in Escherichia coli are controlled by a complex regulatory system that involves transcription of the rne gene from three promoters
Authors/Creators: M.C. Ow (Author/Creator) - University of Georgia
Q. Liu (Author/Creator) - University of Georgia
B.K. Mohanty (Author/Creator) - University of Georgia
M.E. Andrew (Author/Creator) - University of Georgia
V.F. Maples (Author/Creator) - University of Georgia
S.R. Kushner (Author/Creator) - *For correspondence. E-mail skushner@arches.uga.edu; Tel. (+1) 706 542 8000; Fax (+1) 706 542 3910.
Publication Details: Molecular Microbiology, Vol.43(1), pp.159-171
Publisher: Blackwell Publishing
Identifiers: 991005540270107891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.42 Bacteriology; 1.42.131 Bacterial Gene Regulation
Web Of Science research areas: Biochemistry & Molecular Biology; Microbiology
ESI research areas: Microbiology