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Journal article
RPS27L Enhances Myogenesis and Muscle Mass by Targeting IGF1 Through Liquid‐Liquid Phase Separation
Advanced science, e12354
2025
PMID: 40886325
Abstract
RNA‐binding proteins (RBPs) play a pivotal role in post‐transcriptional regulation of gene expression, critically influencing skeletal myogenesis, muscle growth, and regeneration. Despite the recent identification of RBP Rps27l (ribosomal protein S27‐like) as a regulator affecting myogenic proliferation and differentiation, its functions and regulatory mechanisms in skeletal muscle development remain largely unknown. In this study, it is observed that muscle‐specific Rps27l knock‐in (M─KI) mice exhibit significantly increased muscle mass, enlarged myofiber size, a higher proportion of fast‐twitch myofibers, and enhanced muscle regeneration capabilities compared to wild‐type controls. Overexpression of Rps27l promotes myoblast proliferation while inhibiting differentiation in skeletal muscle cells. Mechanistically, it is revealed that the expression of Rps27l is negatively regulated by SIX4, a myogenic transcription factor. The N‐terminal intrinsically disordered region of RPS27L facilitates liquid‐liquid phase separation (LLPS) and interacts with IGF1 to collaboratively regulate myogenesis. The findings uncover the novel regulatory roles of RPS27L in skeletal muscle and highlight the significance of RPS27L‐driven LLPS in myogenesis.
Details
- Title
- RPS27L Enhances Myogenesis and Muscle Mass by Targeting IGF1 Through Liquid‐Liquid Phase Separation
- Authors/Creators
- Xiaoqin Liu - Agricultural Genomics Institute at ShenzhenYilong Yao - Agricultural Genomics Institute at ShenzhenJunyu Yan - Agricultural Genomics Institute at ShenzhenMu Zeng - Chinese Academy of Agricultural SciencesXinhao Fan - Agricultural Genomics Institute at ShenzhenYijie Tang - Agricultural Genomics Institute at ShenzhenJiju Li - Agricultural Genomics Institute at ShenzhenYanwen Liu - Agricultural Genomics Institute at ShenzhenShanying Yan - Agricultural Genomics Institute at ShenzhenWei Wang - Agricultural Genomics Institute at ShenzhenLijuan Chen - Chinese Academy of Agricultural SciencesRuipu Chen - Agricultural Genomics Institute at ShenzhenYuxin Huang - Agricultural Genomics Institute at ShenzhenHonor Calnan - Murdoch University, Centre for Animal Production and HealthHeng Wang - Shandong Agricultural UniversityGraham Gardner - Murdoch University, Centre for Animal Production and HealthYalan Yang - Chinese Academy of Agricultural SciencesZhonglin Tang - Shenzhen Branch Guangdong Laboratory of Lingnan Modern Agriculture Key Laboratory of Livestock and Poultry Multi‐omics of MARA Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518124 China, Kunpeng Institute of Modern Agriculture at Foshan Agricultural Genomics Institute Chinese Academy of Agricultural Sciences Foshan 528226 China
- Publication Details
- Advanced science, e12354
- Publisher
- Advanced Science published by Wiley-VCH GmbH; HOBOKEN
- Number of pages
- 16
- Identifiers
- 991005810349507891
- Copyright
- ©2025 The Author(s).
- Murdoch Affiliation
- College of Environmental and Life Sciences; School of Veterinary Medicine; Centre for Animal Production and Health
- Language
- English
- Resource Type
- Journal article
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