Rapid and Self-Administrable Capillary Blood Microsampling Demonstrates Statistical Equivalence with Standard Venous Collections in NMR-Based Lipoprotein Analysis

Jayden Lee Roberts; Nathan G Lawler; Julien Wist; Elaine Holmes; Luke Whiley; Philipp Nitschke; Nicola Gray Dr; Reika Masuda

doi:10.1021/acs.analchem.3c05152

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Rapid and Self-Administrable Capillary Blood Microsampling Demonstrates Statistical Equivalence with Standard Venous Collections in NMR-Based Lipoprotein Analysis

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Rapid and Self-Administrable Capillary Blood Microsampling Demonstrates Statistical Equivalence with Standard Venous Collections in NMR-Based Lipoprotein Analysis

Jayden Lee Roberts, Nathan G Lawler, Julien Wist, Elaine Holmes, Luke Whiley, Philipp Nitschke, Nicola Gray Dr and Reika Masuda

Analytical Chemistry, Vol.96(11), pp.4505-4513

2024

DOI: https://doi.org/10.1021/acs.analchem.3c05152

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Abstract

NMR spectroscopy

lipoproteins

We investigated plasma and serum blood derivatives from capillary blood microsamples (500 μL, MiniCollect tubes) and corresponding venous blood (10 mL vacutainers). Samples from 20 healthy participants were analyzed by 1 H NMR, and 112 lipoprotein subfraction parameters; 3 supramolecular phospholipid composite (SPC) parameters from SPC 1 , SPC 2 , and SPC 3 subfractions; 2 N-acetyl signals from α-1-acid glycoprotein (Glyc), GlycA, and GlycB; and 3 calculated parameters, SPC (total), SPC 3 /SPC 2 , and Glyc (total) were assessed. Using linear regression between capillary and venous collection sites, we explained that agreement (Adj. R 2 ≥ 0.8, p < 0.001) was witnessed for 86% of plasma parameters (103/120) and 88% of serum parameters (106/120), indicating that capillary lipoprotein, SPC, and Glyc concentrations follow changes in venous concentrations. These results indicate that capillary blood microsamples are suitable for sampling in remote areas and for high-frequency longitudinal sampling of the majority of lipoproteins, SPCs, and Glycs.

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Title: Rapid and Self-Administrable Capillary Blood Microsampling Demonstrates Statistical Equivalence with Standard Venous Collections in NMR-Based Lipoprotein Analysis
Authors/Creators: Jayden Lee Roberts
Nathan G Lawler (Corresponding Author) - Murdoch University, Health Futures Institute
Julien Wist (Author) - Murdoch University, Centre for Computational and Systems Medicine
Elaine Holmes (Author) - Murdoch University, Centre for Computational and Systems Medicine
Luke Whiley (Author) - Murdoch University, Centre for Computational and Systems Medicine
Philipp Nitschke (Author) - Murdoch University
Nicola Gray Dr (Author) - Murdoch University, Centre for Computational and Systems Medicine
Reika Masuda (Author) - Murdoch University
Publication Details: Analytical Chemistry, Vol.96(11), pp.4505-4513
Publisher: ACS Publications
Identifiers: 991005638670207891
Copyright: © 2024 The Authors.
Murdoch Affiliation: Health Futures Institute; Centre for Computational and Systems Medicine
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 2 Chemistry; 2.211 Mass Spectrometry; 2.211.990 Metabolomics
Web Of Science research areas: Chemistry, Analytical
ESI research areas: Chemistry