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Rapid detection of H and R Panton–Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis
Journal article   Peer reviewed

Rapid detection of H and R Panton–Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

S.Y.C. Tong, R.A. Lilliebridge, D.C. Holt, G.W. Coombs, B.J. Currie and P.M. Giffard
Diagnostic Microbiology and Infectious Disease, Vol.67(4), pp.399-401
2010
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Abstract

We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC).

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Collaboration types
Domestic collaboration
Citation topics
1 Clinical & Life Sciences
1.23 Antibiotics & Antimicrobials
1.23.173 MRSA and VRE
Web Of Science research areas
Infectious Diseases
Microbiology
ESI research areas
Immunology
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