Journal article
Rapid isolation of DNA from fresh and preserved fish scales for polymerase chain reaction
Marine Biotechnology, Vol.3(3), pp.199-204
2001
Abstract
We developed a simple and inexpensive method to extract DNA from fresh and preserved fish scales. The procedure is based on boiling the scales in 5% Chelex 100, followed by digestion with proteinase K and subsequent absorption of genomic DNA using silica. A single fresh scale from larger species (e.g., tilapia) or a few scales from smaller species (e.g., 4 scales from zebrafish) provide over 200 ng of DNA, enough for at least 40 polymerase chain reaction amplifications. The procedure is applicable for DNA isolation not only from fresh and ethanol-preserved scales, but also from dried and formaldehyde-treated samples, and thus might be useful for investigating specimens stored in museums and other collections. Since the removal of a few scales is a gentle means of sample collection, this technique will allow analysis of genetic diversity, mating systems, and parentage in populations of endangered or ornamental fish with minimal experimental influence.
Details
- Title
- Rapid isolation of DNA from fresh and preserved fish scales for polymerase chain reaction
- Authors/Creators
- G-H Yue (Author/Creator) - National University of SingaporeL. Orbán (Author/Creator) - National University of Singapore
- Publication Details
- Marine Biotechnology, Vol.3(3), pp.199-204
- Publisher
- Springer-Verlag
- Identifiers
- 991005542613407891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
Metrics
37 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.64 Phylogenetics & Genomics
- 3.64.71 Genetic Diversity
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Marine & Freshwater Biology
- ESI research areas
- Plant & Animal Science