Journal article
Rapid screening for the detection of HLA-B57 and HLA-B58 in prevention of drug hypersensitivity
Tissue Antigens, Vol.78(1), pp.11-20
2011
Abstract
HLA-B57 and HLA-B58 are major histocompatibility class (MHC)-I allotypes that are potentially predictive of important clinical immune phenotypes. HLA-B*5701 is strongly associated with hypersensitivity to the HIV drug abacavir, liver toxicity from the antibiotic flucloxacillin and is a marker for slow progression of HIV AIDS. HLA-B*5801 is associated with hypersensitivity to allopurinol used to treat hyperuricaemia and recurrent gout. Here we describe a monoclonal antibody (mAb) specific for HLA-B57 and HLA-B58 that provides an inexpensive and sensitive screen for these MHC-I allotypes. The usefulness of HLA-B57 screening for prediction of abacavir hypersensitivity was shown in three independent laboratories, including confirmation of the mAb sensitivity and specificity in a cohort of patients enrolled in the PREDICT-1 trial. Our data show that patients who test negative by mAb screening comprise 90%-95% of all individuals in most human populations and require no further human leukocyte antigen (HLA) typing. Patients who test positive by mAb screening should proceed to high-resolution typing to ascertain the presence of HLA-B*5701 or HLA-B*5801. Hence, mAb screening provides a low-cost alternative to high-resolution typing of all patients and lends itself to point-of-care diagnostics and rapid ascertainment of low-risk patients who can begin immediate therapy with abacavir, flucloxacillin or allopurinol.
Details
- Title
- Rapid screening for the detection of HLA-B57 and HLA-B58 in prevention of drug hypersensitivity
- Authors/Creators
- L. Kostenko (Author/Creator) - The University of MelbourneL. Kjer-Nielsen (Author/Creator) - The University of MelbourneI. Nicholson (Author/Creator) - Australian Red Cross LifebloodF. Hudson (Author/Creator) - Australian Red Cross LifebloodA. Lucas (Author/Creator) - Murdoch UniversityB. Foley (Author/Creator) - Murdoch UniversityK. Chen (Author/Creator) - San Francisco General HospitalK. Lynch (Author/Creator) - San Francisco General HospitalJ. Nguyen (Author/Creator) - San Francisco General HospitalA.H.B. Wu (Author/Creator)B.D. Tait (Author/Creator) - Australian Red Cross LifebloodR. Holdsworth (Author/Creator) - Australian Red Cross LifebloodS. Mallal (Author/Creator) - Murdoch UniversityJ. Rossjohn (Author/Creator) - Monash UniversityM. Bharadwaj (Author/Creator) - The University of MelbourneJ. McCluskey (Author/Creator) - The University of Melbourne
- Publication Details
- Tissue Antigens, Vol.78(1), pp.11-20
- Publisher
- Blackwell Publishing Inc.
- Identifiers
- 991005542939107891
- Copyright
- © 2011 John Wiley & Sons A/S
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.265 Dermatology - Skin Allergies
- 1.265.1140 Drug Hypersensitivity
- Web Of Science research areas
- Cell Biology
- Immunology
- Pathology
- ESI research areas
- Molecular Biology & Genetics