Journal article
Rapid separation of seed gliadins by reversed-phase ultra performance liquid chromatography (RP-UPLC) and its application in wheat cultivar and germplasm identification
Bioscience, Biotechnology, and Biochemistry, Vol.79(5), pp.808-815
2015
Abstract
To separate gliadin from wheat flour, a novel and stability-indicating reversed-phase ultra performance liquid chromatography (RP-UPLC) method is established and optimized. A comparative analysis of routine capillary electrophoresis (CE), reversed-phase high-performance liquid chromatography (RP-HPLC), and RP-UPLC was performed and the results showed that the resolution and efficiency of RP-UPLC were significantly higher than those of CE and RP-HPLC. Characteristic RP-UPLC patterns of different bread wheat variety and related species were readily identified. These results demonstrated that our RP-UPLC procedure resulted in significant improvements in sensitivity, speed, and resolution, and thus is highly useful in wheat cultivar and germplasm identification.
The novel UP-UPLC method showing high resolution and efficiency in the separation of seed gliadins might be applied to wheat variety and germplasm identification.
Details
- Title
- Rapid separation of seed gliadins by reversed-phase ultra performance liquid chromatography (RP-UPLC) and its application in wheat cultivar and germplasm identification
- Authors/Creators
- C. Han (Author/Creator)X. Lu (Author/Creator)Z. Yu (Author/Creator)X. Li (Author/Creator)W. Ma (Author/Creator)Y. Yan (Author/Creator)
- Publication Details
- Bioscience, Biotechnology, and Biochemistry, Vol.79(5), pp.808-815
- Publisher
- Taylor & Francis
- Identifiers
- 991005542435007891
- Copyright
- © 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.99 Cereal Starch Properties
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology
- Chemistry, Applied
- Food Science & Technology
- ESI research areas
- Biology & Biochemistry