Journal article
Rapidly mutating Y-STR analyses of compromised forensic samples
International Journal of Legal Medicine, Vol.132(2), pp.397-403
2018
Abstract
Rapidly mutating Y-chromosomal short tandem repeats (RM Y-STRs) were identified to improve differentiation of unrelated males and also to enable separating closely and distantly related males in human identity testing in forensic and other applications. RM-Yplex assay was developed as a single multiplex that is capable of simultaneously amplifying all currently known RM Y-STRs, and reproducibility and sensitivity testing were performed on reference samples. Additional analyses are necessary to test its suitability for analysing compromised forensic samples. For this purpose, we applied the RM-Yplex assay to approximately 70-year-old skeletons that were used as a model for poorly preserved, challenging forensic samples. We analysed 57 male skeletal remains (bones and teeth) from 55 skeletons excavated from the Second World War (WWII) mass graves in Slovenia. The RM-Yplex typing was successful in all 57 samples; there were 56% full profiles obtained, and in partial profiles, up to 7 locus drop-outs were observed and they appeared correlated with low DNA quantities and degradation of DNA obtained from WWII bone and tooth samples. The longest loci, DYS403S1b, DYS547, DYS627 and DYS526b, were the most often dropped-out RM Y-STRs. In spite of high frequency of drop-out events, the RM-Yplex typing was successful in all WWII samples, showing the possibility of successful amplification of at least half of the RM Y-STRs even from the most compromised samples analysed.
Details
- Title
- Rapidly mutating Y-STR analyses of compromised forensic samples
- Authors/Creators
- R. Alghafri (Author/Creator) - General Department of Forensic Sciences and Criminology, Dubai Police General Head QuartersI. Zupanič Pajnič (Author/Creator)T. Zupanc (Author/Creator) - University of LjubljanaJ. Balažic (Author/Creator) - University of LjubljanaP. Shrivastava (Author/Creator) - Central Forensic Science Laboratory
- Publication Details
- International Journal of Legal Medicine, Vol.132(2), pp.397-403
- Publisher
- Springer Verlag
- Identifiers
- 991005542133607891
- Copyright
- © 2020 Springer Nature Switzerland AG.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
24 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.189 Genome Studies
- 1.189.310 Population Genetics
- Web Of Science research areas
- Medicine, Legal
- ESI research areas
- Clinical Medicine