Journal article
Rat interleukin-4 assays
Journal of Immunological Methods, Vol.221(1-2), pp.141-150
1998
Abstract
The present article describes procedures to measure rat IL-4 protein. The RT-PCR technique has been successfully and widely used to measure IL-4 mRNA, but it does not determine IL-4 protein synthesis. Assays to measure rat IL-4 protein based on its biological activity were developed using the mAb OX-81, which inhibits rat IL-4 activity. Two bioassays were attempted based on the ability of IL-4 to induce the proliferation of T cell blasts and to increase MHC class II expression on resting B cells. A second mAb against rat IL-4 was used in a sandwich ELISA to detect rat IL-4. This ELISA is satisfactory although its sensitivity is not as high as that of the bioassay. According to our experience, the bioassay based on the induction of class II MHC molecules on B cells is the technique of choice for rat IL-4 determination because it proved specific, sensitive and reproducible.
Details
- Title
- Rat interleukin-4 assays
- Authors/Creators
- F. Ramírez (Author/Creator) - University of OxfordP.A. Stumbles (Author/Creator) - University of OxfordM. Puklavec (Author/Creator) - University of OxfordD. Mason (Author/Creator) - University of Oxford
- Publication Details
- Journal of Immunological Methods, Vol.221(1-2), pp.141-150
- Publisher
- Elsevier BV
- Identifiers
- 991005543228707891
- Copyright
- 1998 Elsevier Science B.V.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.127 T Cell Regulation
- Web Of Science research areas
- Biochemical Research Methods
- Immunology
- ESI research areas
- Immunology