Real-time PCR for identification of five species of Cryptolestes based on COI barcode region

D. Chen; T. Zhang; R. Aulicky; V. Stejskal; Y. Ren; Y. Cao; D. Hawthorne; Z. Li

doi:10.1016/j.jspr.2020.101623

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Real-time PCR for identification of five species of Cryptolestes based on COI barcode region

Journal article

Peer reviewed

Real-time PCR for identification of five species of Cryptolestes based on COI barcode region

D. Chen, T. Zhang, R. Aulicky, V. Stejskal, Y. Ren, Y. Cao, D. Hawthorne and Z. Li

Journal of Stored Products Research, Vol.87, Art. 101623

2020

DOI: https://doi.org/10.1016/j.jspr.2020.101623

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Abstract

Cryptolestes (Coleoptera: Laemophloeidae) are economically important pests, which damage stored products in granaries and mills. Because it is difficult to distinguish Cryptolestes species morphologically, we develop here a high sensitivity real-time PCR method for distinguishing five species of Cryptolestes (C. ferrugineus, C. pusillus, C. turcicus, C. pusilloides and C. capensis). The lower limit of DNA concentration required for species identification is 0.1 ng/μl for C. ferruginues and C. pusilloides, and 0.01 ng/μl for C. pusillus, C. turcicus, and C. capensis. This method successfully determined species assignments for previously unidentified Cryptolestes spp. Collected from the field and will facilitate Cryptolestes identification in the field of plant quarantine and stored product protection.

Details

Title: Real-time PCR for identification of five species of Cryptolestes based on COI barcode region
Authors/Creators: D. Chen (Author/Creator) - China Agricultural University
T. Zhang (Author/Creator) - Academy of National Food and Strategic Reserves Administration, Beijing, 100037, China
R. Aulicky (Author/Creator) - Czech Agrifood Research Center
V. Stejskal (Author/Creator) - Czech Agrifood Research Center
Y. Ren (Author/Creator) - Murdoch University
Y. Cao (Author/Creator) - Academy of National Food and Strategic Reserves Administration, Beijing, 100037, China
D. Hawthorne (Author/Creator) - University of Maryland, College Park
Z. Li (Author/Creator) - China Agricultural University
Publication Details: Journal of Stored Products Research, Vol.87, Art. 101623
Publisher: Elsevier BV
Identifiers: 991005541364307891
Copyright: © 2020 Elsevier Ltd.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.32 Entomology; 3.32.1138 Botanical Insecticides
Web Of Science research areas: Entomology
ESI research areas: Plant & Animal Science