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Published (Version of Record)CC BY V4.0, Open Access
Journal article
Real-time fluorometric isothermal LAMP assay for detection of Chlamydia pecorum in rapidly processed ovine abortion samples: A veterinary practitioner’s perspective
Pathogens, Vol.10(9), Article 1157
2021
Abstract
Traditional methods of detecting Chlamydia pecorum in tissue samples such as polymerase chain reaction or cell culture are laborious and costly. We evaluated the use of a previously developed C. pecorum LAMP assay using minimally processed ovine samples. Cotyledon (n = 16), foetal liver (n = 22), foetal lung (n = 2), and vaginal (n = 6) swabs, in addition to cotyledon (n = 6) and foetal liver (n = 8) tissue samples, were rapidly processed and used for LAMP testing without DNA extraction. Overall, LAMP test results were highly congruent with the in-house reference qPCR, with 80.43% (37/46; 72.73% positive agreement (PA); 84.75% negative agreement (NA)) overall agreeance for swab samples, and 85.71% (12/14; 80% PA; 88.89% NA) overall agreeance for tissue samples. Out of the 11 total discrepant results, discrepancy was mainly observed in samples (n = 10) with less than 100 copies/µL C. pecorum DNA. While sensitivity could be improved, the simplicity, low cost, and accuracy of detection makes this test amenable for use at point-of-care for detecting C. pecorum in sheep.
Details
- Title
- Real-time fluorometric isothermal LAMP assay for detection of Chlamydia pecorum in rapidly processed ovine abortion samples: A veterinary practitioner’s perspective
- Authors/Creators
- T. Clune (Author/Creator)S. Anstey (Author/Creator)V. Kasimov (Author/Creator)C. Jacobson (Author/Creator)M. Jelocnik (Author/Creator)
- Publication Details
- Pathogens, Vol.10(9), Article 1157
- Publisher
- MDPI
- Identifiers
- 991005541862807891
- Copyright
- © 2021 by the authors
- Murdoch Affiliation
- Centre for Animal Production and Health
- Language
- English
- Resource Type
- Journal article
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.248 Sexually Transmitted Infections
- 1.248.655 Chlamydia Infections
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology