Recombinant Jembrana disease virus gag proteins identify several different antigenic domains but do not facilitate serological differentiation of JDV and nonpathogenic bovine lentiviruses

M. Desport; M.E. Stewart; C.A. Sheridan; W.G.F. Ditcham; S. Setiyaningsih; W.M. Tenaya; N. Hartaningsih; G.E. Wilcox

doi:10.1016/j.jviromet.2004.11.015

Back

Recombinant Jembrana disease virus gag proteins identify several different antigenic domains but do not facilitate serological differentiation of JDV and nonpathogenic bovine lentiviruses

Journal article

Peer reviewed

Recombinant Jembrana disease virus gag proteins identify several different antigenic domains but do not facilitate serological differentiation of JDV and nonpathogenic bovine lentiviruses

M. Desport, M.E. Stewart, C.A. Sheridan, W.G.F. Ditcham, S. Setiyaningsih, W.M. Tenaya, N. Hartaningsih and G.E. Wilcox

Journal of Virological Methods, Vol.124(1-2), pp.135-142

2005

DOI: https://doi.org/10.1016/j.jviromet.2004.11.015

Files and links (1)

url

Link to Published Version *Subscription may be requiredView

Abstract

In Indonesia, it is suspected that there are two bovine lentiviruses circulating in the cattle population: a pathogenic Jembrana disease virus (JDV), and a nonpathogenic bovine immunodeficiency-like virus (BIV). Both viruses cross-react antigenically and cannot be differentiated by current serological tests using JDV antigens. To identify possible type-specific epitopes, a series of recombinant protein constructs including the matrix, capsid and nucleocapsid proteins were produced from JDV gag and the expressed proteins were tested by Western blot using JDV and BIV hyperimmune sera. JDV matrix and truncated capsid proteins were recognised by both JDV and BIV hyperimmune sera indicating that there were multiple cross-reactive epitopes present in JDV gag. At least three epitopic regions were identified in these constructs, including the major homology region, by monoclonal antibody binding studies. JDV nucleocapsid recombinant protein was not recognised by either JDV or BIV hyperimmune sera and none of the recombinant gag proteins were able to differentiate between JDV positive sera from Jembrana disease endemic and Jembrana disease-free areas. Additionally, a 40 amino acid recombinant subunit protein encompassing the region recently found to contain an epitope unique to BIV [Zheng, L., Zhang, S., Wood, C., Kapil, S., Wilcox, G.E., Loughin, T.A., Minocha, H.C., 2001. Differentiation of two bovine lentiviruses by a monoclonal antibody on the basis of epitope specificity. Clin. Diagn. Lab. Immunol. 8, 283-287] was tested but was not recognised by either JDV positive sera from Jembrana disease-endemic or Jembrana disease-free areas.

Details

Title: Recombinant Jembrana disease virus gag proteins identify several different antigenic domains but do not facilitate serological differentiation of JDV and nonpathogenic bovine lentiviruses
Authors/Creators: M. Desport (Author/Creator)
M.E. Stewart (Author/Creator)
C.A. Sheridan (Author/Creator)
W.G.F. Ditcham (Author/Creator)
S. Setiyaningsih (Author/Creator)
W.M. Tenaya (Author/Creator)
N. Hartaningsih (Author/Creator)
G.E. Wilcox (Author/Creator)
Publication Details: Journal of Virological Methods, Vol.124(1-2), pp.135-142
Publisher: Elsevier BV
Identifiers: 991005543329807891
Copyright: © 2004 Elsevier B.V.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

28 Record Views

13 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.66 HIV; 1.66.46 HIV Pathogenesis
Web Of Science research areas: Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology
ESI research areas: Microbiology