Journal article
Reduced volume for direct PCR amplification of blood reference samples using Identifiler® Direct and Globalfiler™ Express assays
Forensic Science International: Genetics Supplement Series, Vol.6, pp.e340-e341
2017
Abstract
FTA® cards were developed for analysing reference samples such as blood or saliva and helping to store the sample for long term purposes as it kills the microorganisms that comes along with biological stains which can be potential inhibitors. The aim of this research is to optimize a reduced volume PCR amplification for STR analysis of reference blood samples. This approach will help to reduce the costs of DNA profiling using direct amplification multiplex assays. A total number of 130 blood samples from different individuals were collected on FTA® cards. The reaction was optimized using half volume for amplification with Identifiler® Direct and Globalfiler™ Express multiplex assays. The protocol was applied in a forensic laboratory and was able to reduce costs and account for variability of DNA amounts among reference samples.
Details
- Title
- Reduced volume for direct PCR amplification of blood reference samples using Identifiler® Direct and Globalfiler™ Express assays
- Authors/Creators
- R. Almheiri (Author/Creator) - General Department of Forensic Sciences and Criminology, Dubai Police G.H.Q., Dubai, United Arab EmiratesR. Alghafri (Author/Creator) - United Arab Emirates University
- Publication Details
- Forensic Science International: Genetics Supplement Series, Vol.6, pp.e340-e341
- Publisher
- Elsevier
- Identifiers
- 991005542230907891
- Copyright
- © 2017 Elsevier B.V.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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