Journal article
Regulation of KIR3DL3 Expression via miRNA
Genes, Vol.10(8)
2019
Abstract
Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes. Although the structure of KIR3DL3 is suggestive of an inhibitory receptor, the function of KIR3DL3 has not been demonstrated and cognate ligands have not been identified. KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells, but cell surface expression of KIR3DL3 cannot be detected. Accordingly, post-transcriptional regulation of KIR3DL3 should exist. Using bioinformatics analysis, we identified three candidate micro ribonucleic acids (miRNAs; miR-26a-5p, -26b-5p and -185-5p) that potentially regulate KIR3DL3 expression. Luciferase reporter assays utilizing constructs with mutated miRNA-binding sites of miR-26a-5p, -26b-5p and -185-5p in the 3’-untranslated region (3’ UTR) of KIR3DL3 resulted in up-regulation of luciferase activity demonstrating a potential mechanism of gene regulation. Furthermore, knockdown of the same endogenous miRNAs using silencing ribonucleic acid (siRNA) led to induced surface expression of KIR3DL3. In conclusion, we provide a novel mechanism of functional regulation of KIR3DL3 via miRNAs. These findings are relevant in understanding the generation of KIR repertoire and NK cell clonality
Details
- Title
- Regulation of KIR3DL3 Expression via miRNA
- Authors/Creators
- R. Nutalai (Author/Creator) - Khon Kaen UniversityS. Gaudieri (Author/Creator) - Vanderbilt University Medical CenterA. Jumnainsong (Author/Creator) - Khon Kaen UniversityC. Leelayuwat (Author/Creator) - Khon Kaen University
- Publication Details
- Genes, Vol.10(8)
- Publisher
- MDPI
- Identifiers
- 991005545168907891
- Copyright
- © 2019 MDPI
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.1021 Natural Killer Cells
- Web Of Science research areas
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics