Regulation of sarcoma cell migration, invasion and invadopodia formation by AFAP1L1 through a phosphotyrosine-dependent pathway

S.R. Tie; D.J. McCarthy; T.S. Kendrick; A. Louw; C. Le; J. Satiaputra; N. Kucera; M. Phillips; E. Ingley

doi:10.1038/onc.2015.272

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Regulation of sarcoma cell migration, invasion and invadopodia formation by AFAP1L1 through a phosphotyrosine-dependent pathway

Journal article

Peer reviewed

Regulation of sarcoma cell migration, invasion and invadopodia formation by AFAP1L1 through a phosphotyrosine-dependent pathway

S.R. Tie, D.J. McCarthy, T.S. Kendrick, A. Louw, C. Le, J. Satiaputra, N. Kucera, M. Phillips and E. Ingley

Oncogene, Vol.35(16), pp.2098-2111

2016

DOI: https://doi.org/10.1038/onc.2015.272

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Abstract

Invasion and metastasis are controlled by the invadopodia, which delivers matrix-degrading enzymes to the invasion interface permitting cancer cell penetration and spread into healthy tissue. We have identified a novel pathway that directs Lyn/Src family tyrosine kinase signals to the invadopodia to regulate sarcoma cell invasion via the molecule AFAP-1-like-1 (AFAP1L1), a new member of the AFAP (actin filament-associated protein) family. We show that AFAP1L1 can transform cells, promote migration and co-expression with active Lyn profoundly influences cell morphology and movement. AFAP1L1 intersects several invadopodia pathway components through its multiple domains and motifs, including the following (i) pleckstrin homology domains that bind phospholipids generated at the plasma membrane by phosphoinositide 3-kinase, (ii) a direct filamentous-actin binding domain and (iii) phospho-tyrosine motifs (pY136 and pY566) that specifically bind Vav2 and Nck2 SH2 domains, respectively. These phosphotyrosine motifs are essential for AFAP1L1-mediated cytoskeleton regulation. Through its interaction with Vav2, AFAP1L1 regulates Rac activity and downstream control of PAK1/2/3 (p21-activated kinases) phosphorylation of myosin light chain (MLC) kinase and MLC2. AFAP1L1 interaction with Nck2 recruits actin-nucleating complexes. Significantly, in osteosarcoma cell lines, knockdown of AFAP1L1 inhibits phosphorylated MLC2 recruitment to filamentous-actin structures, disrupts invadopodia formation, cell attachment, migration and invasion. These data define a novel pathway that directs Lyn/Src family tyrosine kinase signals to sarcoma cell invadopodia through specific recruitment of Vav2 and Nck2 to phosphorylated AFAP1L1, to control cell migration and invasion.

Details

Title: Regulation of sarcoma cell migration, invasion and invadopodia formation by AFAP1L1 through a phosphotyrosine-dependent pathway
Authors/Creators: S.R. Tie (Author/Creator)
D.J. McCarthy (Author/Creator)
T.S. Kendrick (Author/Creator)
A. Louw (Author/Creator)
C. Le (Author/Creator)
J. Satiaputra (Author/Creator)
N. Kucera (Author/Creator)
M. Phillips (Author/Creator)
E. Ingley (Author/Creator)
Publication Details: Oncogene, Vol.35(16), pp.2098-2111
Publisher: Nature Publishing Group
Identifiers: 991005544368007891
Copyright: © 2016 Macmillan Publishers Limited. A
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.96 Cell Biology; 1.96.224 Cell Mechanics
Web Of Science research areas: Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity; Oncology
ESI research areas: Molecular Biology & Genetics