Abstract
To reveal the feedbacks and regulating mechanisms of microplastic types and doses on microbial community, a microcosm experiment was carried out with two non-degradable microplastics [polyethylene (PE) and polyvinyl chloride (PVC)] and four biodegradable microplastics [poly(butylene succinate) (PBS), polyhydroxyalkanoates (PHA), poly(butyleneadipate-co-terephthalate) (PBAT), and polypropylene carbonate (PPC)] at different levels (1%, 7%, and 28%). As a result, the content of total carbon (TC), soil organic carbon (SOC), and microbial biomass carbon (MBC) (expect MBC in PBS soil) increased with increasing doses of microplastics, and increased at the lowest PE dose rate. Biodegradable microplastics created a more active ecological niche while enriching more pathogens than non-degradable microplastics. Structural equation modeling indicated that microbial diversities were in a type-dependent assembly, whereas microbial compositions were more profoundly affected by the microplastic doses, ultimately. The standardized total effect coefficient of microplastic types on bacterial and fungal diversities was -0.429 and -0.282, and that of doses on bacterial and fungal compositions was 0.487 and 0.336, respectively. Both microplastic types and doses significantly impacted pH, electrical conductivity, total nitrogen, TC, SOC, and MBC, subsequently inhibiting microbial diversities and stimulating microbial compositions with particular pathways. The results provide a comprehensive understanding for evaluating the potential risk of microplastics.
Microplastics, being almost ubiquitous in terrestrial ecosystems, were charged with affecting soil ecological function and biogeochemical cycling. However, there is a knowledge gap in understanding the regulating mechanisms of microplastic types and doses on microbial communities and ecological functions. This study indicates that bacterial and fungal diversities are in a type-dependent assembly, whereas microbial community structures are more profoundly affected by the doses of microplastics. These results illustrate the disturbance behavior of different microplastic types and doses on the soil ecology in the microcosm, providing a comprehensive understanding for evaluating the potential risk of microplastics.
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•MPs inhibited microbial diversities while stimulated microbial compositions.•Bacterial and fungal diversities were in a microplastic-type-dependent assembly.•Microbial community structures had a more profound response to microplastic doses.•MPs regulated microbial community by altering soil physicochemical properties.•Biodegradable MPs created a more active ecological niche while enriching pathogens.