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Relationship between paraoxonase 1 activity and HDL concentration during naturally occurring babesiosis in dogs
Journal article   Peer reviewed

Relationship between paraoxonase 1 activity and HDL concentration during naturally occurring babesiosis in dogs

G. Rossi, J. Kuleš, R.B. Rafaj, V. Mrljak, A. Giordano and S. Paltrinieri
Veterinary Clinical Pathology, Vol.42(4), E31
2013
DOI: https://doi.org/10.1111/vcp.12091
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Abstract

Paraoxonase 1 (PON1) is a negative acute phase protein that circulates in blood bound to high density lipoproteins (HDLs) and exerts an antioxidant function. During systemic inflammation, the Reactive Oxygen Species (ROS) produced against pathogens leads to lipid peroxidation converting HDL to LDL. Consequently, PON1 serum activity decreases due to an increased consumption and detachment from HDL. The aim of this study was to assess the antioxidant role of PON1 towards HDL, using naturally infected dogs with babesiosis, a disease associated with changes of serum HDL concentrations, before and after treatment. To this aim, serum from 30 dogs infected by Babesia canis were sampled at admission (day 0) prior to a single treatment with imidocarb dipropionate, and on days 1 and 6. A group of 15 control dogs was also included in the study to assess the actual presence of altered HDL and PON1 at day 0. PON1 and HDL were measured on all the samples using methods validated for canine serum. Compared with controls, serum concentrations of HDL and PON1 activity were significantly lower at day 0. The concentration of HDL significantly increased on days 1 and 6 compared with day 0 (P < .05 for both), and without significant differences between days 1 and 6. PON1 activity did not differ between day 0 and day 1, but significantly increased on day 6 compared with day 0 and day 1 (P < .001 for both). These results support the hypothesis that PON1 exerts an antioxidant role towards HDL also in dogs. The low serum concentration of HDL at admission is consistent with lipid peroxidation. The earlier increase of HDL (day 1) compared to PON1 (day 6) is probably dependent on increased consumption of PON1 on day 1 to reduce the oxidized lipids and to quickly restore the concentration of HDL.

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