Release of beta-casomorphins during in-vitro gastrointestinal digestion of reconstituted milk after heat treatment

D.D. Nguyen; F. Busetti; G. Smolenski; S.K. Johnson; V.A. Solah

doi:10.1016/j.lwt.2020.110312

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Release of beta-casomorphins during in-vitro gastrointestinal digestion of reconstituted milk after heat treatment

Journal article

Peer reviewed

Release of beta-casomorphins during in-vitro gastrointestinal digestion of reconstituted milk after heat treatment

D.D. Nguyen, F. Busetti, G. Smolenski, S.K. Johnson and V.A. Solah

LWT, Vol.136, 110312

2021

DOI: https://doi.org/10.1016/j.lwt.2020.110312

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Abstract

This study assessed the impact of heat treatment on beta-casomorphin 5 (β-CM5) and beta-casomorphin 7 (β-CM7) after in-vitro gastrointestinal digestion of milk representing beta-casein (β-CN) A1A1, A2A2 and A2I phenotype. After heat treatment at 73 °C/20 s, 85 °C/5 min and 121 °C/12 min, milk samples were subjected to in-vitro gastrointestinal digestion. β-CM5/7 were analysed using ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and further confirmed using ultra high-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry (UHPL-HRMS). β-CM5 was not released after in-vitro gastrointestinal digestion of all heated milk. Similarly, β-CM7 was not released in all milk with β-CN A2A2 phenotype. However, this peptide level ranged from 127.25 to 198.10 ng/mL (4.94–7.70 ng/mg protein) in heated milk with β-CN A1A1 phenotype, whereas it was released at much lower levels ranging from 19.35 to 24.50 ng/mL (0.71–0.91 ng/mg protein) in heated milk with β-CN A2I phenotype.

Details

Title: Release of beta-casomorphins during in-vitro gastrointestinal digestion of reconstituted milk after heat treatment
Authors/Creators: D.D. Nguyen (Author/Creator)
F. Busetti (Author/Creator) - Edith Cowan University
G. Smolenski (Author/Creator) - MS3 Solutions Ltd, Ruakura Research Centre, Hamilton, 3214, New Zealand
S.K. Johnson (Author/Creator) - Curtin University
V.A. Solah (Author/Creator) - Vietnam National University Ho Chi Minh City
Publication Details: LWT, Vol.136, 110312
Publisher: Elsevier Ltd.
Identifiers: 991005540486607891
Murdoch Affiliation: School of Medical, Molecular and Forensic Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.784 Dairy Product Chemistry
Web Of Science research areas: Food Science & Technology
ESI research areas: Agricultural Sciences