Journal article
Release of beta-casomorphins during in-vitro gastrointestinal digestion of reconstituted milk after heat treatment
LWT, Vol.136, 110312
2021
Abstract
This study assessed the impact of heat treatment on beta-casomorphin 5 (β-CM5) and beta-casomorphin 7 (β-CM7) after in-vitro gastrointestinal digestion of milk representing beta-casein (β-CN) A1A1, A2A2 and A2I phenotype. After heat treatment at 73 °C/20 s, 85 °C/5 min and 121 °C/12 min, milk samples were subjected to in-vitro gastrointestinal digestion. β-CM5/7 were analysed using ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and further confirmed using ultra high-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry (UHPL-HRMS). β-CM5 was not released after in-vitro gastrointestinal digestion of all heated milk. Similarly, β-CM7 was not released in all milk with β-CN A2A2 phenotype. However, this peptide level ranged from 127.25 to 198.10 ng/mL (4.94–7.70 ng/mg protein) in heated milk with β-CN A1A1 phenotype, whereas it was released at much lower levels ranging from 19.35 to 24.50 ng/mL (0.71–0.91 ng/mg protein) in heated milk with β-CN A2I phenotype.
Details
- Title
- Release of beta-casomorphins during in-vitro gastrointestinal digestion of reconstituted milk after heat treatment
- Authors/Creators
- D.D. Nguyen (Author/Creator)F. Busetti (Author/Creator) - Edith Cowan UniversityG. Smolenski (Author/Creator) - MS3 Solutions Ltd, Ruakura Research Centre, Hamilton, 3214, New ZealandS.K. Johnson (Author/Creator) - Curtin UniversityV.A. Solah (Author/Creator) - Vietnam National University Ho Chi Minh City
- Publication Details
- LWT, Vol.136, 110312
- Publisher
- Elsevier Ltd.
- Identifiers
- 991005540486607891
- Copyright
- © 2020 Elsevier Ltd.
- Murdoch Affiliation
- School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
Metrics
60 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.784 Dairy Product Chemistry
- Web Of Science research areas
- Food Science & Technology
- ESI research areas
- Agricultural Sciences