Journal article
Reproducible regeneration of callus from suspension culture protoplasts of the grass lolium multiflorum
Zeitschrift für Pflanzenphysiologie, Vol.105(3), pp.267-274
1982
Abstract
Protoplasts have been isolated from suspension cultures that originated from single immature embryos of Italian ryegrass (Lolium multiflorum). Conditions required for efficient division and colony formation by these protoplasts have been investigated. Up to 50 % of the protoplasts developed walls and divided at low auxin (0.25 mg · 1-1 NAA; 2,4-D; IAA) and low cytokinin (0.25 mg · 1 -1, BAP) levels, but higher auxin levels were required for sustained cell division and colony formation. Culture of callus lines derived from protoplasts has not yet led to the regeneration of plants, although plants could be regenerated from one of the original suspension culture lines. The results are discussed with reference to the culture of other gramineous protoplasts.
Details
- Title
- Reproducible regeneration of callus from suspension culture protoplasts of the grass lolium multiflorum
- Authors/Creators
- M.G.K. Jones (Author/Creator) - Aberystwyth UniversityP.J. Dale (Author/Creator) - Aberystwyth University
- Publication Details
- Zeitschrift für Pflanzenphysiologie, Vol.105(3), pp.267-274
- Publisher
- Elsevier
- Identifiers
- 991005544193407891
- Copyright
- © 1982 Gustav Fischer Verlag, Stuttgart.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
42 Record Views