Journal article
Retinal amino acid neurochemistry of the southern hemisphere lamprey, Geotria australis
PLoS ONE, Vol.8(3), e58406
2013
Abstract
Lampreys are one of the two surviving groups of the agnathan (jawless) stages in vertebrate evolution and are thus ideal candidates for elucidating the evolution of visual systems. This study investigated the retinal amino acid neurochemistry of the southern hemisphere lamprey Geotria australis during the downstream migration of the young, recently-metamorphosed juveniles to the sea and during the upstream migration of the fully-grown and sexually-maturing adults to their spawning areas. Glutamate and taurine were distributed throughout the retina, whilst GABA and glycine were confined to neurons of the inner retina matching patterns seen in most other vertebrates. Glutamine and aspartate immunoreactivity was closely matched to Müller cell morphology. Between the migratory phases, few differences were observed in the distribution of major neurotransmitters i.e. glutamate, GABA and glycine, but changes in amino acids associated with retinal metabolism i.e. glutamine and aspartate, were evident. Taurine immunoreactivity was mostly conserved between migrant stages, consistent with its role in primary cell functions such as osmoregulation. Further investigation of glutamate signalling using the probe agmatine (AGB) to map cation channel permeability revealed entry of AGB into photoreceptors and horizontal cells followed by accumulation in inner retinal neurons. Similarities in AGB profiles between upstream and downstream migrant of G. australis confirmed the conservation of glutamate neurotransmission. Finally, calcium binding proteins, calbindin and calretinin were localized to the inner retina whilst recoverin was localized to photoreceptors. Overall, conservation of major amino acid neurotransmitters and calcium-associated proteins in the lamprey retina confirms these elements as essential features of the vertebrate visual system. On the other hand, metabolic elements of the retina such as neurotransmitter precursor amino acids and Müller cells are more sensitive to environmental changes associated with migration.
Details
- Title
- Retinal amino acid neurochemistry of the southern hemisphere lamprey, Geotria australis
- Authors/Creators
- L. Nivison-Smith (Author/Creator) - UNSW SydneyS.P. Collin (Author/Creator) - The University of Western AustraliaY. Zhu (Author/Creator) - UNSW SydneyS. Ready (Author/Creator) - University of AucklandM.L. Acosta (Author/Creator) - University of AucklandD.M. Hunt (Author/Creator) - The University of Western AustraliaI.C. Potter (Author/Creator) - Murdoch UniversityM. Kalloniatis (Author/Creator) - University of Auckland
- Publication Details
- PLoS ONE, Vol.8(3), e58406
- Publisher
- Public Library of Science
- Identifiers
- 991005540053007891
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.36 Ophthalmology
- 1.36.212 Genetic Retinopathies
- Web Of Science research areas
- Neurosciences
- ESI research areas
- Neuroscience & Behavior