Journal article
Reversible control by vitamin D of granulocytes and bacteria in the lungs of mice: An ovalbumin-induced model of allergic airway disease
PLoS ONE, Vol.8(6), e67823
2013
Abstract
Vitamin D may be essential for restricting the development and severity of allergic diseases and asthma, but a direct causal link between vitamin D deficiency and asthma has yet to be established. We have developed a 'low dose' model of allergic airway disease induced by intraperitoneal injection with ovalbumin (1 μg) and aluminium hydroxide (0.2 mg) in which characteristics of atopic asthma are recapitulated, including airway hyperresponsiveness, antigen-specific immunoglobulin type-E and lung inflammation. We assessed the effects of vitamin D deficiency throughout life (from conception until adulthood) on the severity of ovalbumin-induced allergic airway disease in vitamin D-replete and -deficient BALB/c mice using this model. Vitamin D had protective effects such that deficiency significantly enhanced eosinophil and neutrophil numbers in the bronchoalveolar lavage fluid of male but not female mice. Vitamin D also suppressed the proliferation and T helper cell type-2 cytokine-secreting capacity of airway-draining lymph node cells from both male and female mice. Supplementation of initially vitamin D-deficient mice with vitamin D for four weeks returned serum 25-hydroxyvitamin D to levels observed in initially vitamin D-replete mice, and also suppressed eosinophil and neutrophil numbers in the bronchoalveolar lavage fluid of male mice. Using generic 16 S rRNA primers, increased bacterial levels were detected in the lungs of initially vitamin D-deficient male mice, which were also reduced by vitamin D supplementation. These results indicate that vitamin D controls granulocyte levels in the bronchoalveolar lavage fluid in an allergen-sensitive manner, and may contribute towards the severity of asthma in a gender-specific fashion through regulation of respiratory bacteria.
Details
- Title
- Reversible control by vitamin D of granulocytes and bacteria in the lungs of mice: An ovalbumin-induced model of allergic airway disease
- Authors/Creators
- S. Gorman (Author/Creator)C.E. Weeden (Author/Creator)D.H.W. Tan (Author/Creator)N.M. Scott (Author/Creator)J. Hart (Author/Creator)R.E. Foong (Author/Creator)D. Mok (Author/Creator)N. Stephens (Author/Creator)G. Zosky (Author/Creator)P.H. Hart (Author/Creator)
- Publication Details
- PLoS ONE, Vol.8(6), e67823
- Publisher
- Public Library of Science
- Identifiers
- 991005545254307891
- Copyright
- © 2013 Gorman et al
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
- Note
- This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.80 Bone Diseases
- 1.80.279 Vitamin D
- Web Of Science research areas
- Immunology
- ESI research areas
- Immunology