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Published (Version of Record)CC BY V4.0, Open Access
Journal article
Root-to-shoot long-distance mobile miRNAs identified from Nicotiana rootstocks
International Journal of Molecular Sciences, Vol.22(23), Article 12821
2021
Abstract
Root-derived mobile signals play critical roles in coordinating a shoot’s response to underground conditions. However, the identification of root-to-shoot long-distance mobile signals has been scant. In this study, we aimed to characterize root-to-shoot endogenous mobile miRNAs by using an Arabidopsis/Nicotiana interfamilial heterograft in which these two taxonomically distant species with clear genetic backgrounds had sufficient diversity in differentiating miRNA sources. Small RNA deep sequencing analysis revealed that 82 miRNAs from the Arabidopsis scion could travel through the graft union to reach the rootstock, whereas only a very small subset of miRNA (6 miRNAs) preferred the root-to-shoot movement. We demonstrated in an ex vivo RNA imaging experiment that the root-to-shoot mobile Nb-miR164, Nb-miR395 and Nb-miR397 were targeted to plasmodesmata using the bacteriophage coat protein MS2 system. Furthermore, the Nb-miR164 was shown to move from the roots to the shoots to induce phenotypic changes when its overexpressing line was used as rootstock, strongly supporting that root-derived Nb-miR164 was able to modify the scion trait via its long-distance movement.
Details
- Title
- Root-to-shoot long-distance mobile miRNAs identified from Nicotiana rootstocks
- Authors/Creators
- Z. Deng (Author/Creator)H. Wu (Author/Creator)D. Li (Author/Creator)L. Li (Author/Creator)Z. Wang (Author/Creator)W. Yuan (Author/Creator)Y. Xing (Author/Creator)C. Li (Author/Creator)D. Liang (Author/Creator)
- Publication Details
- International Journal of Molecular Sciences, Vol.22(23), Article 12821
- Publisher
- MDPI
- Identifiers
- 991005540042507891
- Copyright
- © 2021 by the authors
- Murdoch Affiliation
- Western Barley Genetics Alliance
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.159 Arabidopsis
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry