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SCIMP is a spatiotemporal transmembrane scaffold for Erk1/2 to direct pro-inflammatory signaling in TLR-activated macrophages
Journal article   Open access   Peer reviewed

SCIMP is a spatiotemporal transmembrane scaffold for Erk1/2 to direct pro-inflammatory signaling in TLR-activated macrophages

R.M. Lucas, L. Liu, J.E.B. Curson, Y.W.H. Koh, N. Tuladhar, N.D. Condon, K. Das Gupta, S.S. Burgener, K. Schroder, E. Ingley, …
Cell Reports, Vol.36(10), Art. 109662
2021
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Abstract

Immune cells are armed with Toll-like receptors (TLRs) for sensing and responding to pathogens and other danger cues. The role of extracellular-signal-regulated kinases 1/2 (Erk1/2) in TLR signaling remains enigmatic, with both pro- and anti-inflammatory functions described. We reveal here that the immune-specific transmembrane adaptor SCIMP is a direct scaffold for Erk1/2 in TLR pathways, with high-resolution, live-cell imaging revealing that SCIMP guides the spatial and temporal recruitment of Erk2 to membrane ruffles and macropinosomes for pro-inflammatory TLR4 signaling. SCIMP-deficient mice display defects in Erk1/2 recruitment to TLR4, c-Fos activation, and pro-inflammatory cytokine production, with these effects being phenocopied by Erk1/2 signaling inhibition. Our findings thus delineate a selective role for SCIMP as a key scaffold for the membrane recruitment of Erk1/2 kinase to initiate TLR-mediated pro-inflammatory responses in macrophages.

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Collaboration types
Domestic collaboration
Citation topics
1 Clinical & Life Sciences
1.6 Immunology
1.6.609 Toll-like Receptors
Web Of Science research areas
Cell Biology
ESI research areas
Molecular Biology & Genetics
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