SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley

Xifeng Ren; Jibin Wang; Lipan Liu; Genlou Sun; Chengdao Li; Hong Luo; Dongfa Sun

doi:10.1038/srep31741

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SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley

Journal article

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SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley

Xifeng Ren, Jibin Wang, Lipan Liu, Genlou Sun, Chengdao Li, Hong Luo and Dongfa Sun

Scientific reports, Vol.6(1), 31741

2016

DOI: https://doi.org/10.1038/srep31741

PMCID: PMC4987670

PMID: 27530597

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Abstract

Agricultural genetics

Centromeres

Plant breeding

A high-density linkage map is a valuable tool for functional genomics and breeding. A newly developed sequence-based marker technology, restriction site associated DNA (RAD) sequencing, has been proven to be powerful for the rapid discovery and genotyping of genome-wide single nucleotide polymorphism (SNP) markers and for the high-density genetic map construction. The objective of this research was to construct a high-density genetic map of barley using RAD sequencing. 1894 high-quality SNP markers were developed and mapped onto all seven chromosomes together with 68 SSR markers. These 1962 markers constituted a total genetic length of 1375.8 cM and an average of 0.7 cM between adjacent loci. The number of markers within each linkage group ranged from 209 to 396. The new recessive dwarfing gene btwd1 in Huaai 11 was mapped onto the high density linkage maps. The result showed that the btwd1 is positioned between SNP marks 7HL_6335336 and 7_249275418 with a genetic distance of 0.9 cM and 0.7 cM on chromosome 7H, respectively. The SNP-based high-density genetic map developed and the dwarfing gene btwd1 mapped in this study provide critical information for position cloning of the btwd1 gene and molecular breeding of barley.

Details

Title: SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley
Authors/Creators: Xifeng Ren - Plant (United States)
Jibin Wang - Huazhong Agricultural University
Lipan Liu - Huazhong Agricultural University
Genlou Sun - Saint Mary's University
Chengdao Li - Department of Agriculture &Food/Agricultural Research Western Australia, 3 Baron-Hay Court, South Perth, WA 6155, Australia.
Hong Luo - Huazhong Agricultural University
Dongfa Sun - Huazhong Agricultural University
Publication Details: Scientific reports, Vol.6(1), 31741
Publisher: NATURE PORTFOLIO
Number of pages: 7
Grant note: CARS-5 / Earmarked Fund for China Agriculture Research System 2662016PY048 / Fundamental Research Funds for the Central Universities 31301310 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC)
Identifiers: 991005570078807891
Murdoch Affiliation: Centre for Crop and Food Innovation
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Genetics & Heredity
ESI research areas: Multidisciplinary