STRmix™ collaborative exercise on DNA mixture interpretation

J-A Bright; K. Cheng; Z. Kerr; C. McGovern; H. Kelly; T.R. Moretti; M.A. Smith; F.R. Bieber; B. Budowle; M.D. Coble; R. Alghafri; P.S. Allen; A. Barber; V. Beamer; C. Buettner; M. Russell; C. Gehrig; T. Hicks; J. Charak; K. Cheong-Wing; A. Ciecko; C.T. Davis; M. Donley; N. Pedersen; B. Gartside; D. Granger; M.M. Greer-Ritzheimer; E. Reisinger; J. Kennedy; E. Grammer; M. Kaplan; D. Hansen; H.J. Larsen; A. Laureano; C. Li; E. Lien; E. Lindberg; C. Kelly; B. Mallinder; S. Malsom; A. Yacovone-Margetts; A. McWhorter; S.M. Prajapati; T. Powell; G. Shutler; K. Stevenson; A.R. Stonehouse; L. Smith; J. Murakami; E. Halsing; D. Wright; L. Clarke; D.A. Taylor; J. Buckleton

doi:10.1016/j.fsigen.2019.01.006

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STRmix™ collaborative exercise on DNA mixture interpretation

Journal article

Peer reviewed

STRmix™ collaborative exercise on DNA mixture interpretation

J-A Bright, K. Cheng, Z. Kerr, C. McGovern, H. Kelly, T.R. Moretti, M.A. Smith, F.R. Bieber, B. Budowle, M.D. Coble, …

Forensic Science International: Genetics, Vol.40, pp.1-8

2019

DOI: https://doi.org/10.1016/j.fsigen.2019.01.006

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Abstract

An intra and inter-laboratory study using the probabilistic genotyping (PG) software STRmix™ is reported. Two complex mixtures from the PROVEDIt set, analysed on an Applied Biosystems™ 3500 Series Genetic Analyzer, were selected. 174 participants responded. For Sample 1 (low template, in the order of 200 rfu for major contributors) five participants described the comparison as inconclusive with respect to the POI or excluded him. Where LRs were assigned, the point estimates ranging from 2 × 104 to 8 × 106. For Sample 2 (in the order of 2000 rfu for major contributors), LRs ranged from 2 × 1028 to 2 × 1029. Where LRs were calculated, the differences between participants can be attributed to (from largest to smallest impact): • varying number of contributors (NoC), • the exclusion of some loci within the interpretation, • differences in local CE data analysis methods leading to variation in the peaks present and their heights in the input files used, • and run-to-run variation due to the random sampling inherent to all MCMC-based methods. This study demonstrates a high level of repeatability and reproducibility among the participants. For those results that differed from the mode, the differences in LR were almost always minor or conservative.

Details

Title: STRmix™ collaborative exercise on DNA mixture interpretation
Authors/Creators: J-A Bright (Author/Creator)
K. Cheng (Author/Creator)
Z. Kerr (Author/Creator)
C. McGovern (Author/Creator)
H. Kelly (Author/Creator)
T.R. Moretti (Author/Creator) - Federal Bureau of Investigation
M.A. Smith (Author/Creator) - Federal Bureau of Investigation
F.R. Bieber (Author/Creator) - Brigham and Women's Hospital
B. Budowle (Author/Creator) - University of North Texas Health Science Center
M.D. Coble (Author/Creator) - University of North Texas Health Science Center
R. Alghafri (Author/Creator)
P.S. Allen (Author/Creator)
A. Barber (Author/Creator)
V. Beamer (Author/Creator)
C. Buettner (Author/Creator)
M. Russell (Author/Creator)
C. Gehrig (Author/Creator) - American Board of Legal Medicine
T. Hicks (Author/Creator) - University of Lausanne
J. Charak (Author/Creator)
K. Cheong-Wing (Author/Creator)
A. Ciecko (Author/Creator)
C.T. Davis (Author/Creator)
M. Donley (Author/Creator)
N. Pedersen (Author/Creator) - Victoria Police
B. Gartside (Author/Creator)
D. Granger (Author/Creator)
M.M. Greer-Ritzheimer (Author/Creator)
E. Reisinger (Author/Creator)
J. Kennedy (Author/Creator)
E. Grammer (Author/Creator)
M. Kaplan (Author/Creator)
D. Hansen (Author/Creator)
H.J. Larsen (Author/Creator) - University of Copenhagen
A. Laureano (Author/Creator)
C. Li (Author/Creator)
E. Lien (Author/Creator)
E. Lindberg (Author/Creator)
C. Kelly (Author/Creator)
B. Mallinder (Author/Creator)
S. Malsom (Author/Creator)
A. Yacovone-Margetts (Author/Creator)
A. McWhorter (Author/Creator)
S.M. Prajapati (Author/Creator)
T. Powell (Author/Creator)
G. Shutler (Author/Creator) - Washington State Patrol
K. Stevenson (Author/Creator)
A.R. Stonehouse (Author/Creator)
L. Smith (Author/Creator) - United States Department of the Army
J. Murakami (Author/Creator)
E. Halsing (Author/Creator) - California Department of Justice
D. Wright (Author/Creator)
L. Clarke (Author/Creator)
D.A. Taylor (Author/Creator) - Flinders University
J. Buckleton (Author/Creator) - University of Auckland
Publication Details: Forensic Science International: Genetics, Vol.40, pp.1-8
Publisher: Elsevier BV
Identifiers: 991005542262007891
Copyright: © 2019 Elsevier B.V.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Industry collaboration; Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.189 Genome Studies; 1.189.310 Population Genetics
Web Of Science research areas: Genetics & Heredity; Medicine, Legal
ESI research areas: Molecular Biology & Genetics