Journal article
Sampling, storage and laboratory approaches for dissolved organic matter characterisation in freshwaters: Moving from nutrient fraction to molecular-scale characterisation
Science of The Total Environment, Vol.827, Art. 154105
2022
Abstract
Recent research has highlighted the importance of dissolved organic matter (DOM) for ecosystem function and because of this paradigm shift, it has become crucial to not only quantify its contribution to river nutrient loads but also to characterise its composition. There has been a significant research effort utilising optical methods, such as fluorescence and UV–Vis spectrophotometry, in order to start exploring DOM character. However, these methods still lack the granularity to understand the chemical composition at the molecular level, which is vital to properly understanding its functional role in freshwater ecosystems. As a direct result, there has been a shift towards including molecular-scale analyses to investigate the in-stream processing of the material. Alongside this, recent methodological advancements, particularly in mass spectrometry are opening new opportunities for probing one of the most complex environmental mixtures. However, in order to fully exploit these opportunities, it is key that the way that samples are collected, processed and stored is considered carefully such that sample integrity is maintained. There are additional challenges when collecting water samples for analysis at molecular scale, for example the ultra-low concentrations of individual compounds within DOM means that the samples are sensitive to contamination. This paper discusses current sample collection, processing and storage protocols for this C, N and P quantification and characterisation in freshwaters, and proposes a new standardised protocol suitable for both nutrient fraction quantification and molecular scale analyses, based on method development and testing undertaken in our UK Natural Environment Research Council large grant programme, characterising the nature, origins and ecological significance of Dissolved Organic Matter IN freshwater Ecosystems (DOMAINE).
Details
- Title
- Sampling, storage and laboratory approaches for dissolved organic matter characterisation in freshwaters: Moving from nutrient fraction to molecular-scale characterisation
- Authors/Creators
- C.E.M. Lloyd (Author/Creator) - University of BristolP.J. Johnes (Author/Creator) - University of BristolJ.A. Pemberton (Author/Creator) - Wessex WaterC.A. Yates (Author/Creator) - University of BristolD. Jones (Author/Creator) - Bangor UniversityR.P. Evershed (Author/Creator) - University of Bristol
- Publication Details
- Science of The Total Environment, Vol.827, Art. 154105
- Publisher
- Elsevier BV
- Identifiers
- 991005542864407891
- Copyright
- © 2022 Elsevier B.V.
- Murdoch Affiliation
- Centre for Sustainable Farming Systems; Food Futures Institute
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.45 Soil Science
- 3.45.1049 Dissolved Organic Matter
- Web Of Science research areas
- Environmental Sciences
- ESI research areas
- Environment/Ecology