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CC BY-NC V4.0, Open Access
Journal article
ScRNA-seq reveals dark- and light-induced differentially expressed gene atlases of seedling leaves in Arachis hypogaea L
Plant biotechnology journal, Vol.22(7), pp.1848-1866
2024
PMID: 38391124
Abstract
Although the regulatory mechanisms of dark and light-induced plant morphogenesis have been broadly investigated, the biological process in peanuts has not been systematically explored on single-cell resolution. Herein, 10 cell clusters were characterized using scRNA-seq-identified marker genes, based on 13 409 and 11 296 single cells from 1-week-old peanut seedling leaves grown under dark and light conditions. 6104 genes and 50 transcription factors (TFs) displayed significant expression patterns in distinct cell clusters, which provided gene resources for profiling dark/light-induced candidate genes. Further pseudo-time trajectory and cell cycle evidence supported that dark repressed the cell division and perturbed normal cell cycle, especially the PORA abundances correlated with 11 TFs highly enriched in mesophyll to restrict the chlorophyllide synthesis. Additionally, light repressed the epidermis cell developmental trajectory extending by inhibiting the growth hormone pathway, and 21 TFs probably contributed to the different genes transcriptional dynamic. Eventually, peanut AHL17 was identified from the profile of differentially expressed TFs, which encoded protein located in the nucleus promoted leaf epidermal cell enlargement when ectopically overexpressed in Arabidopsis through the regulatory phytohormone pathway. Overall, our study presents the different gene atlases in peanut etiolated and green seedlings, providing novel biological insights to elucidate light-induced leaf cell development at the single-cell level.
Details
- Title
- ScRNA-seq reveals dark- and light-induced differentially expressed gene atlases of seedling leaves in Arachis hypogaea L
- Authors/Creators
- Quanqing Deng - Guangdong Academy of Agricultural SciencesPuxuan Du - Guangdong Academy of Agricultural SciencesSunil S Gangurde - SEMIYanbin Hong - Guangdong Academy of Agricultural SciencesYuan Xiao - Wannan Medical CollegeDongxiu Hu - Guangdong Academy of Agricultural SciencesHaifen Li - Guangdong Academy of Agricultural SciencesQing Lu - Guangdong Academy of Agricultural SciencesShaoxiong Li - Guangdong Academy of Agricultural SciencesHaiyan Liu - Guangdong Academy of Agricultural SciencesRunfeng Wang - Guangdong Academy of Agricultural SciencesLu Huang - Guangdong Academy of Agricultural SciencesWenyi Wang - College of Agriculture, South China Agricultural University, Guangzhou, Guangdong Province, ChinaVanika Garg - Murdoch UniversityXuanqiang Liang - Guangdong Academy of Agricultural SciencesRajeev K Varshney - College of Agriculture, South China Agricultural University, Guangzhou, Guangdong Province, ChinaXiaoping Chen - Guangdong Academy of Agricultural SciencesHao Liu - Guangdong Academy of Agricultural Sciences
- Publication Details
- Plant biotechnology journal, Vol.22(7), pp.1848-1866
- Publisher
- John Wiley & Sons, Inc.
- Grant note
- 202101 / Wellcome Trust
- Identifiers
- 991005639364807891
- Copyright
- © 2024 The Authors.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre; Centre for Crop and Food Innovation; Food Futures Institute
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.159 Arabidopsis
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Plant Sciences
- ESI research areas
- Plant & Animal Science