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Journal article
Screening Plant Growth-Promoting Bacteria with Antimicrobial Properties for Upland Rice
Journal of microbiology and biotechnology, Vol.34(5), pp.1029-1039
2024
Abstract
This study explores beneficial bacteria isolated from the roots and rhizosphere soil of Khao Rai Leum Pua Phetchabun rice plants. A total of 315 bacterial isolates (KK001 to KK315) were obtained. Plant growth-promoting traits (phosphate solubilization and indole-3-acetic acid (IAA) production), and antimicrobial activity against three rice pathogens (Curvularia lunata NUF001, Bipolaris oryzae 2464, and Xanthomonas oryzae pv. oryzae) were assessed. KK074 was the most prolific in IAA production, generating 362.6 ± 28.0 μg/ml, and KK007 excelled in tricalcium phosphate solubilization, achieving 714.2 ± 12.1 μg/ml. In antimicrobial assays using the dual culture method, KK024 and KK281 exhibited strong inhibitory activity against C. lunata, and KK269 was particularly effective against B. oryzae. In the evaluation of antimicrobial metabolite production, KK281 and KK288 exhibited strong antifungal activities in cell-free supernatants. Given the superior performance of KK281, taxonomically identified as Bacillus sp. KK281, it was investigated further. Lipopeptide extracts from KK281 had significant antimicrobial activity against C. lunata and a minimum inhibitory concentration (MIC) of 3.1 mg/ml against X. oryzae pv. oryzae. LC-ESI-MS/MS analysis revealed the presence of surfactin in the lipopeptide extract. The crude extract was non-cytotoxic to the L-929 cell line at tested concentrations. In conclusion, the in vitro plant growth-promoting and disease-controlling attributes of Bacillus sp. KK281 make it a strong candidate for field evaluation to boost plant growth and manage disease in upland rice.
Details
- Title
- Screening Plant Growth-Promoting Bacteria with Antimicrobial Properties for Upland Rice
- Authors/Creators
- Khammool Khamsuk - Naresuan UniversityBernard DellWasu Pathom-Aree - Chiang Mai UniversityWanwarang Pathaichindachote - Naresuan UniversityNungruthai Suphrom - Naresuan UniversityNareeluk Nakaew - Naresuan UniversityJuangjun Jumpathong - Naresuan University
- Publication Details
- Journal of microbiology and biotechnology, Vol.34(5), pp.1029-1039
- Publisher
- The Korean Society for Microbiology and Biotechnology
- Identifiers
- 991005672968907891
- Copyright
- © 2024 by the authors.
- Murdoch Affiliation
- Centre for Crop and Food Innovation
- Language
- English
- Resource Type
- Journal article
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.556 Microbial Biocontrol
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology
- ESI research areas
- Microbiology