Secreted biofilm factors adversely affect cellular wound healing responses in vitro

Robert Marano; Hilary Wallace; Dulharie Wijeratne; Mark W Fear; Hui San Wong; Ryan O'Handley

doi:10.1038/srep13296

Back

Secreted biofilm factors adversely affect cellular wound healing responses in vitro

Journal article

Open access

Peer reviewed

Secreted biofilm factors adversely affect cellular wound healing responses in vitro

Robert Marano, Hilary Wallace, Dulharie Wijeratne, Mark W Fear, Hui San Wong and Ryan O'Handley

Scientific reports, Vol.5(1), 13296

2015

DOI: https://doi.org/10.1038/srep13296

PMID: 26278131

Files and links (1)

pdf

Published3.26 MBDownload View

CC BY V4.0, Open Access

Abstract

Multidisciplinary Sciences

Science & Technology

Science & Technology - Other Topics

Although most chronic wounds possess an underlying pathology, infectious agents also contribute. In many instances, pathogens exist as biofilms forming clusters surrounded by a secreted extracellular substance. We hypothesized that compounds secreted by biofilm bacteria may inhibit normal wound healing events including cell proliferation and migration. Conditioned media from two common bacterial species associated with chronic skin wounds and chronic tympanic membrane perforations, Staphylococcus aureus and Pseudomonas aeruginosa, were evaluated for their capacity to affect keratinocyte proliferation and migration. Additionally, proteomic analysis was performed to identify proteins within the biofilm conditioned media that may contribute to these observed effects. Biofilm conditioned media from both species inhibited proliferation in human tympanic membrane derived keratinocytes, whereas only biofilm conditioned media from S. aureus inhibited migration. Human epidermal keratinocytes were found to be more sensitive to the effects of the conditioned media resulting in high levels of cell death. Heat treatment and microfiltration suggested that S. aureus activity was due to a protein, while P. aeruginosa activity was more likely due to a small molecule. Proteomic analysis identified several proteins with putative links to delayed wound healing. These include alpha hemolysin, alcohol dehydrogenase, fructose-bisphosphate aldolase, lactate dehydrogenase and epidermal cell differentiation inhibitor.

Details

Title: Secreted biofilm factors adversely affect cellular wound healing responses in vitro
Authors/Creators: Robert Marano - The University of Western Australia
Hilary Wallace - The University of Western Australia
Dulharie Wijeratne - The University of Western Australia
Mark W Fear - The University of Western Australia
Hui San Wong - Murdoch University, School of Medical, Molecular and Forensic Sciences
Ryan O'Handley - Univ Adelaide, Sch Anim & Vet Sci, Adelaide, SA, Australia
Publication Details: Scientific reports, Vol.5(1), 13296
Publisher: NATURE PORTFOLIO; BERLIN
Number of pages: 11
Identifiers: 991005806650107891
Murdoch Affiliation: School of Medical, Molecular and Forensic Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

9 File views/ downloads

10 Record Views

42 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.42 Bacteriology; 1.42.567 Quorum Sensing
Web Of Science research areas: Cell Biology
ESI research areas: Clinical Medicine