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CC BY V4.0, Open Access
Journal article
Selection and evaluation of lactic acid bacteria from chicken feces in Thailand as potential probiotics
PeerJ (San Francisco, CA), Vol.11, e16637
2023
PMID: 38107571
Abstract
Lactic acid bacteria (LAB) are widely used as probiotics in poultry production due to their resilience to low pH and high bile salt concentrations, as well as their beneficial effects on growth performance and antagonistic activity against enteric pathogens. However, the efficacy of probiotics depends on strain selection and their ability to colonize the host's intestine. This study aimed to select, identify, and evaluate LAB strains isolated from chicken feces in Thailand for potential use as probiotics in the chicken industry.
LAB strains were isolated from 58 pooled fresh fecal samples collected from chicken farms in various regions of Thailand, including commercial and backyard farms. Gram-positive rods or cocci with catalase-negative characteristics from colonies showing a clear zone on MRS agar supplemented with 0.5% CaCO
were identified using MALDI-TOF mass spectrometry. The LAB isolates were evaluated for acid (pH 2.5 and pH 4.5) and bile salt (0.3% and 0.7%) tolerance. Additionally, their cell surface properties, resistance to phenol, antimicrobial activity, hemolytic activity, and presence of antimicrobial resistance genes were determined.
A total of 91 LAB isolates belonging to the
, and
genera were obtained from chicken feces samples. Backyard farm feces exhibited a greater LAB diversity compared to commercial chickens. Five strains, including
BF12 and
BF9, BF14, BYF20, and BYF26, were selected based on their high tolerance to acid, bile salts, and phenol.
BF12 and
BF14 demonstrated strong adhesion ability. The five LAB isolates exhibited significant cell-cell interactions (auto-aggregation) and co-aggregation with
. All five LAB isolates showed varying degrees of antimicrobial activity against
strains, with
BYF20 displaying the highest activity. None of the LAB isolates exhibited beta-hemolytic activity. Whole genome analysis showed that
BF12 contained
, and
, whereas
strains BF9 and BF14 carried
, and
.
The selected LAB isolates exhibited basic probiotic characteristics, although some limitations were observed in terms of adhesion ability and the presence of antibiotic resistance genes, requiring further investigation into their genetic location. Future studies will focus on developing a probiotic prototype encapsulation for application in the chicken industry, followed by
evaluations of probiotic efficacy.
Details
- Title
- Selection and evaluation of lactic acid bacteria from chicken feces in Thailand as potential probiotics
- Authors/Creators
- Benjamas Khurajog - Chulalongkorn UniversityYuda Disastra - Chulalongkorn UniversityLum Dau Lawwyne - Chulalongkorn UniversityWandee Sirichokchatchawan - Chulalongkorn UniversityWaree Niyomtham - Chulalongkorn UniversityJitrapa Yindee - Chulalongkorn UniversityDavid John Hampson - School of Veterinary Medicine, Murdoch University, Perth, Western Australia, AustraliaNuvee Prapasarakul - Chulalongkorn University
- Publication Details
- PeerJ (San Francisco, CA), Vol.11, e16637
- Publisher
- PeerJ
- Identifiers
- 991005623667507891
- Copyright
- © 2023 Khurajog et al.
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
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- Citation topics
- 1 Clinical & Life Sciences
- 1.120 Inflammatory Bowel Diseases & Infections
- 1.120.267 Lactic Acid Bacteria
- Web Of Science research areas
- Microbiology
- ESI research areas
- Plant & Animal Science