Journal article
Selection of β-glucosidase producing Bacillus subtilis for Hydrolysis of Ginsenoside from Panax Pseudoginseng
Vietnam Journal of Agricultural Sciences, Vol.19(11), pp.1489-1498
2021
Abstract
The study aimed to select Bacillus subtilis strains which are capable of producing β-glucosidase to hydrolyse ginsenoside from the root of Panax pseudoginseng into the bioactive compound Rg3 and CK. Twenty four strains were primary screened for the β-glucosidase n using 0.1% azo-CMC and 0.3% azo-Avicel. The classification of strains based on the morphology and phylogeny was analysed by the sequence of 16S. β-glucosidase activity obtained from strains was determined by DNS method using PNP-β-D-glucopyranoside substrate. The hydrolysis of ginsenoside to Rg3 with enzyme β-glucosidase was identified using thin layer chromatography (TLC). Rg3 and CK content were determined using high performance liquid chromatography HPLC). Results showed that Bacillus subtilis strain NL812 produced β-glucosidase with the highest activity. The β-glucosidase released from Bacillus subtilis strain NL812 hydrolyzed ginsenoside from Panax pseudoginseng into Rg3 and CK after 30 min. with Rg3 content increased by 50.3% and CK content increased by 47.7%.
Details
- Title
- Selection of β-glucosidase producing Bacillus subtilis for Hydrolysis of Ginsenoside from Panax Pseudoginseng
- Authors/Creators
- Duy Nhan Vu - Vietnam Academy of Science and TechnologyYến Hoàng Thị LêThanh Huyền TrầnDoan Duc NguyenNhu Hương Thị NguyễnHoành Đắc TrịnhTrường Vĩnh Đỗ
- Publication Details
- Vietnam Journal of Agricultural Sciences, Vol.19(11), pp.1489-1498
- Publisher
- Vietnam National University of Agriculture
- Identifiers
- 991005743842507891
- Copyright
- © 2021 VNUA.
- Murdoch Affiliation
- Murdoch University
- Language
- Vietnamese
- Resource Type
- Journal article
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