Journal article
Self DNA perpetuates IPF lung fibroblast senescence in a cGAS-dependent manner
Clinical Science, Vol.134(7), pp.889-905
2020
Abstract
Senescence and mitochondrial stress are mutually reinforcing age-related processes that contribute to idiopathic pulmonary fibrosis (IPF); a lethal disease that manifests primarily in the elderly. Whilst evidence is accumulating that GMP-AMP synthase (cGAS) is crucial in perpetuating senescence by binding damaged DNA released into the cytosol, its role in IPF is not known. The present study examines the contributions of cGAS and self DNA to the senescence of lung fibroblasts from IPF patients (IPF-LFs) and age-matched controls (Ctrl-LFs). cGAS immunoreactivity was observed in regions of fibrosis associated with fibroblasts in lung tissue of IPF patients. Pharmacological inhibition of cGAS or its knockdown by silencing RNA (siRNA) diminished the escalation of IPF-LF senescence in culture over 7 days as measured by decreased p21 and p16 expression, histone 2AXγ phosphorylation and/or IL-6 production (P < 0.05, n = 5–8). The targeting of cGAS also attenuated etoposide-induced senescence in Ctrl-LFs (P < 0.05, n = 5–8). Levels of mitochondrial DNA (mDNA) detected by qPCR in the cytosol and medium of IPF-LFs or senescence-induced Ctrl-LFs were higher than Ctrl-LFs at baseline (P < 0.05, n = 5–7). The addition of DNAse I (100 U/ml) deaccelerated IPF-LF senescence (P < 0.05, n = 5), whereas ectopic mDNA or the induction of endogenous mDNA release augmented Ctrl-LF senescence in a cGAS-dependent manner (P < 0.05, n = 5). In conclusion, we provide evidence that cGAS reinforces lung fibroblast senescence involving damaged self DNA. The targeting of cGAS to supress senescent-like responses may have potential important therapeutic implications in the treatment of IPF.
Details
- Title
- Self DNA perpetuates IPF lung fibroblast senescence in a cGAS-dependent manner
- Authors/Creators
- M. Schuliga (Author/Creator) - University of Newcastle AustraliaJ. Read (Author/Creator) - University of Newcastle AustraliaK.E.C. Blokland (Author/Creator) - University of Newcastle AustraliaD.W. Waters (Author/Creator)J. Burgess (Author/Creator) - University Medical Center GroningenC. Prêle (Author/Creator) - Institute for Respiratory HealthS.E. Mutsaers (Author/Creator) - Institute for Respiratory HealthJ. Jaffar (Author/Creator) - The Alfred HospitalG. Westall (Author/Creator) - The Alfred HospitalA. Reid (Author/Creator) - University of Newcastle AustraliaA. James (Author/Creator) - John Hunter HospitalC. Grainge (Author/Creator) - John Hunter HospitalD.A. Knight (Author/Creator) - Providence Health Care Research Institute
- Publication Details
- Clinical Science, Vol.134(7), pp.889-905
- Publisher
- Portland Press
- Identifiers
- 991005546345807891
- Copyright
- © 2020 Portland Press
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.208 Vasculitis & Autoimmune Disorders
- 1.208.1262 Idiopathic Pulmonary Fibrosis
- Web Of Science research areas
- Medicine, Research & Experimental
- ESI research areas
- Clinical Medicine